Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving glucose or galactose
Patent
1977-12-19
1981-06-02
Naff, David M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving glucose or galactose
435 15, 435 26, 435188, 435190, 435194, C12Q 154, C12N 996
Patent
active
042712645
ABSTRACT:
Stabilized liquid enzyme and coenzyme compositions are prepared for use in biological diagnostic determination of glucose. In one embodiment a composition is prepared containing in admixture an aqueous vehicle, a nicotinamide-adenine dinucleotide coenzyme, a nucleotide, an enzyme including hexokinase and/or glucose-6-phosphate dehydrogenase and an organic solvent to stabilize the enzyme, coenzyme and nucleotide. The compositions have a pH of 6.0 to 8.5 and may contain a bacteriostat such as an azide compound. A preferred organic solvent is a polyol. In an alternative embodiment, a two reagent liquid enzyme and coenzyme composition is prepared wherein the enzyme is stabilized in one solution and the coenzyme and nucleotide stabilized in another solution, and the two solutions combined when used. In the enzyme-containing solution, an ammonia salt can be used as an alternative to the organic solvent to stabilize the enzyme. The compositions exhibit excellent shelf life and the container in which the compositions are stored can be repeatedly opened for use without any substantial degradation of the enzyme, coenzyme and nucleotide.
REFERENCES:
patent: 3539450 (1970-11-01), Deutsch
patent: 3557002 (1971-01-01), McCarty
patent: 3627688 (1971-12-01), McCarty et al.
patent: 3721607 (1973-03-01), Gruber et al.
patent: 3761420 (1973-09-01), Bogardus
patent: 3764478 (1973-10-01), Bergmeyer et al.
patent: 3776900 (1973-12-01), Hammer
patent: 3778350 (1973-12-01), Bergmeyer et al.
George et al., Stabilization of Lactate and Malate Dehydrogenase by Organic Solvents, Biochim. Biophys. Acta., vol. 191, 1969, (pp. 466-468).
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