Stabilized liquid enzyme and coenzyme compositions

Details Stabilized liquid enzyme and coenzyme compositions Stabilized liquid enzyme and coenzyme compositions

1977-12-20

1981-07-07

Naff, David M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435 15, 435 26, 435188, 435190, 435194, C12Q 150, C12N 996

Patent

active

042775620

ABSTRACT:
A multi-reagent for use in stabilized liquid form is prepared containing three separate reagent solutions which can be combined in proper amounts to form a working composition for a biological diagnostic determination. The first reagent solution contains an aqueous vehicle, organic solvent and coenzyme; the second reagent solution contains an aqueous vehicle, organic solvent, enzyme and substrate; and the third reagent solution contains an aqueous vehicle, buffering agent and an additional component such as a sulphydryl compound.

REFERENCES:
patent: 3540984 (1970-11-01), Deutsch
patent: 3557002 (1971-01-01), McCarty
patent: 3627688 (1971-12-01), McCarty et al.
patent: 3764478 (1973-10-01), Bergmeyer et al.
patent: 3776900 (1973-12-01), Hammer
patent: 3778350 (1973-12-01), Bergmeyer et al.
George et al., Stabilization of Lactate and Malate Dehydrogenase by Organic Solvents, Biochim. Biophys. Acta., vol. 191, 1969, (pp. 466-468).

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