Stabilized glucose isomerase enzyme concentrate

Details Stabilized glucose isomerase enzyme concentrate Stabilized glucose isomerase enzyme concentrate

1976-03-19

1978-03-07

Nafe, David M.

Textiles: fiber preparation

195 31F, 195 66R, 195 68, C07G 702, C07G 7028

Patent

active

040778428

ABSTRACT:
A stabilized glucose isomerase concentrate is prepared by contacting an aqueous mixture containing cell-free glucose isomerase and a water miscible organic solvent such as 2-propanol with a magnesium salt to form an enzyme-magnesium precipitate. The cell-free glucose isomerase may be prepared by mixing cells containing intracellular glucose isomerase with the water miscible organic solvent and digesting the cells with a lysozyme enzyme preparation. The stabilized concentrate contains magnesium and the water miscible organic solvent, and retains at least about 95% of its initial isomerase activity when stored at 26.degree. C for up to 30 days and about 80% of its initial isomerase activity when stored at 18.degree. C for up to one year.

REFERENCES:
patent: 3708397 (1973-01-01), Sipos
patent: 3715276 (1973-02-01), Takasaki et al.
patent: 3957587 (1976-05-01), Armbruster et al.

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