Stabilized erythrocytes and methods therefor

Details Stabilized erythrocytes and methods therefor Stabilized erythrocytes and methods therefor

1976-03-16

1979-01-23

Fagelson, Anna P.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Molecular bilayer structure

424 8, 424 11, 424 12, 424 13, 424 75, A61K 3518, A61K 3900, G01N 3316

Patent

active

041361610

ABSTRACT:
A double aldehyde treatment process of erythrocytes is disclosed. Erythrocytes from various sources can be treated in a two-step process to render them stable and subsequently useful in antigen antibody detection systems. Glyoxal is used as a first treating medium followed by a second treatment step using formaldehyde or glyoxal as the fixative. Glyoxal is used in the first step in amounts ranging from 0.1 to 0.4 gm. per 0.8 ml. of Packed Cell Volume of erythrocytes, followed by the second treatment step in which at least 0.1 gm. of formaldehyde or glyoxal and preferably 0.1 to 0.6 gm. per 0.8 ml. Packed Cell Volume of treated erythrocytes is used. The reaction medium is preferably aqueous hypertonic and most preferably a sodium citrate medium. The treated cells can be used in detection of hepatitis associated antigen in a reverse passive hemagglutination test.

REFERENCES:
patent: 3548051 (1970-12-01), Dingwall
patent: 3553310 (1971-01-01), Csizmas
patent: 3708572 (1973-01-01), Peetoon
patent: 3714345 (1973-01-01), Hirata
patent: 3828103 (1974-08-01), Fujita
patent: 3836433 (1974-09-01), Wirth
Ling, Brit. J. of Haematol., vol. 7, 1961, pp. 299-302.
Emmel, Lab Tech in Biol. & Med., Robt. E. Krieger Pub. Co., Huntington, NY, 1970, Facsimile of 4th Ed., 1964, p. 183.

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