Stabilized enzymes and process for preparing them

Details Stabilized enzymes and process for preparing them Stabilized enzymes and process for preparing them

1992-07-27

1995-09-26

Fleisher, Mindy B.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435221, 435219, C12N 950, C12N 954, C12N 956

Patent

active

054533724

ABSTRACT:
Enzymes stabilized against the destabilizing effect of ionic surfactants and a method of producing such stabilized enzymes in which DNA sequences which code for the enzymes are modified by directed mutation in defined positions which correlate with defined surface regions of the enzyme, in such a way that the codon in which the mutation is located now codes for an amino acid differing from the original amino acid.

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Van Der Laan, J. C., et al., Applied and Environmental Microbiology, vol. 57(4), pp. 901-909, Apr. 1991.
Wells, J. A., et al., Trends in Biochemical Sciences, vol. 13, pp. 291.increment.297, Aug. 1988.
Bott, "Modeling and Crystallographic Analysis of Site-Specific Mutants of Subtilisin", World Bio-Tech. Rep. 2, 51-59 (1985).
Russell et al., "Rational Modification of Enzyme Catalysis by Engineering Surface Charge", Nature, 328:496-500 (1987).
Crossin, "Detergency and Biotechnology", Soap/Cosmetics/Chemical Specialities, 64 (4) 48-50 (1988).

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