Stabilized enzymatic solutions and method for determining total

Details Stabilized enzymatic solutions and method for determining total Stabilized enzymatic solutions and method for determining total

1981-10-02

1983-10-11

Castel, Benoit

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving cholesterol

435 19, 435 25, 435 28, 435188, 435810, 436 71, C12Q 160, C12Q 126, C12Q 128, C12N 996

Patent

active

044093268

ABSTRACT:
Stabilized enzymes useful in the diagnostic assay of total cholesterol are prepared by combining three separately prepared reagents: (1) a cholesterol esterase reagent; (2) cholesterol oxidase reagent; and (3) a saline diluent reagent. The cholesterol esterase reagent is prepared by dissolving a salt of cholic acid in a buffer solution providing a pH within the range of about 4 to about 9. To this solution is added a cholesterol esterase. The solution is then mixed with a polyhydroxy organic compound and TRITON-X-100. A cholesterol oxidase and a polyhydroxy organic compound are each dissolved in separate portions of buffer solution to make the cholesterol esterase reagent. The saline diluent solution is prepared by dissolving potassium iodide in water and adding a polyhydroxy organic compound. The resultant three reagents, when combined, provide a stabilized enzyme solution having utility in the total cholesterol assay of a serum sample. The combined reagents have utility in a total oxygen consumption method of analysis of total cholesterol, which analysis method is also disclosed.

REFERENCES:
patent: 3876375 (1975-04-01), Maurukas
patent: 4045296 (1977-08-01), Sternberg
patent: 4143080 (1979-03-01), Harders et al.
patent: 4161425 (1979-07-01), Perry
patent: 4186251 (1980-01-01), Tarbutton
patent: 4212938 (1980-07-01), Gruber et al.
patent: 4226713 (1980-10-01), Goldberg
Allain, C. C. et al., "Enzymatic Determination of Total Serum Cholesterol"; Clinical Chem., vol. 20, No. 4, pp. 470-475 (1974).

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