Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Stablizing an enzyme by forming a mixture – an adduct or a...
Patent
1994-05-10
1996-03-05
Naff, David M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Stablizing an enzyme by forming a mixture, an adduct or a...
135 71, 135 17, 135194, C12N 996, C12N 912, C12Q 150, G01N 3353
Patent
active
054967163
ABSTRACT:
A stabilized liquid enzyme composition is prepared containing at least one enzyme substrate or analog thereof and/or at least one enzyme product or analog thereof combined with the enzyme in a liquid medium containing. The liquid medium may be human, animal or artificial serum. The composition may also contain an activator chosen from a sulfhydryl compound and a divalent metal ion. In a preferred embodiment, the enzyme is isolated creatine kinase MB(CKMB), the substrate may be adenosine triphosphate, its oxidized or analog form, creatine or its analog, and the product may be adenosine diphosphate, its oxidized or analog form or phosphocreatine or its analog. The activator is preferably N-acetyl-cysteine or .beta.-mercaptoethanol. In a preferred embodiment, CKMB is stabilized with a combination of adenosine triphosphate and creatine. A preferred serum is heat-inactivated alkaline-shocked human serum. The composition displays less than 10% loss of CKMB isoenzyme concentration after 27 days storage at room temperature as measured by an immunoassay. The composition is useful as a calibrator or control reference in an immunoassay.
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Abbott Laboratories
Naff David M.
Pope Lawrence S.
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