Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1995-02-10
1996-09-17
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 6, 435 911, 435193, 435810, 536 2433, C07H 2102, C07H 2104, C12Q 168, C12P 1934
Patent
active
055567711
ABSTRACT:
Stabilized reverse transcriptase and RNA polymerase compositions for use in nucleic acid amplification. Compositions are provided for the stabilization of one or more enzymes in a single stabilized formulation. Additional compositions incorporate a dried, stabilized enzyme mixture together with necessary cofactors and enzyme substrates in a single container for use upon rehydration. Also disclosed are methods for making and using stabilized enzyme compositions and kits for nucleic acid amplification incorporating the disclosed compositions of reverse transcriptase and RNA polymerase lyophilized with a cryoprotectant stabilizing agent including trehalose and polyvinylpyrrolidone.
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Davis William M.
Kacian Daniel L.
Putnam James G.
Shen Nancy L.
Fisher Carlos A.
Gen-Probe Incorporated
Tran Paul B.
Zitomer Stephanie W.
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