Stabilized composition, test device, and method for detecting th

Details Stabilized composition, test device, and method for detecting th Stabilized composition, test device, and method for detecting th

1979-11-13

1982-03-09

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving glucose or galactose

23230B, 252408, 422 56, 435 25, 435 28, C12Q 154

Patent

active

043189849

ABSTRACT:
An improved composition, test device and method for detecting the presence of a sugar in a test sample are disclosed. The composition comprises a sugar oxidase, a peroxidase, a chromogen compound capable of producing a detectable response in the presence of hydrogen peroxide and peroxidase, and an enhancer compound having the structure ##STR1## in which the R substituents, same or different, are hydrogen, lower alkyl, lower alkyloxy, aryl or aryloxy; or in which two of the R substituents bound to a common atom together form a closed ring having 3 to about 6 atoms, said ring being saturated, unsaturated or aromatic. R' is hydrogen or an alkyl or alkenyl group having 1 to about 20 carbon atoms. The composition can be incorporated with a carrier matrix to form a test device. The method comprises contacting a test solution with the device and observing any detectable response.

REFERENCES:
patent: 2848308 (1958-08-01), Free
patent: 4071317 (1978-01-01), Lam

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