Stabilized composition of troponin for immunoassays and process

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

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435 71, 435960, 435967, 530841, C07K 14435, C07K 1447, G01N 3353, G01N 33531

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active

055832008

ABSTRACT:
Stabilized composition of troponin I or T for immunoassays, comprising an aqueous solution containing troponin I or troponin T, 1 to 10 molar equivalents of troponin C per molar equivalent of troponin I or T, and Mg.sup.++ and/or Ca.sup.++ ions, as well as a process for stabilizing a composition of troponin I or T for immunoassays consisting in adding from 1 to 10 molar equivalents or troponin C per molar equivalent of troponin I or T.

REFERENCES:
patent: 5290678 (1994-03-01), Jackowski
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Syska, H. and Al., "A new method of preparation of troponin I (inhibitory protein) using affinity chromatography, Evidence for three different forms of troponin I in striated muscle," Febs Letters, vol. 40. No. 2, Apr. 1974, pp. 253-257.
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McCubbin et al. "Physicochemical Studies on the Interaction of the Calcium-Binding Protein (Troponin C) with the Inhibitory Protein (Troponin I) and Calcium Ions" Biochem. 13(13) 2689-2694 1974.
Horwitz et al. "Interaction of Troponin Subunits" J. Biol Chem 254(2) 350-355 1979.
Head et al. "The Interaction of the Calcium-Binding Protein (Troponin C) with Bivalent Cations and the Inhibitory Protein (Troponin I)" Biochem J. 137 145-154 1974.
Larue, Catherine, et al., "New Monoclonal Antibodies as Probes for Human Cardiac Troponin I: Epitopic Analysis with Synthetic Peptides," Molecular Immunology, vol. 29 (2) 271-278 (1992).
Staprans, Ilona, et al., "Skeletal and Cardiac Troponins and Their Components," J. Biochem., vol. 72, (1972), pp. 723-735.
Eisenberg, Evan, et al., "Troponin-Tropomyosin Complex," The Journal of Biological Chemistry, vol. 249, No. 15 (1974), pp. 4742-4748.

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