Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2008-03-03
2011-10-04
Desai, Anand (Department: 1656)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C435S320100, C435S069100, C435S069200, C530S333000, C530S300000, C514S002600
Reexamination Certificate
active
08030464
ABSTRACT:
An intracellular selection system allows screening for peptide bioactivity and stability. Randomized recombinant peptides are screened for bioactivity in a tightly regulated expression system, preferably derived from the wild-type lac operon. Bioactive peptides thus identified are inherently protease- and peptidase-resistant. Also provided are bioactive peptides stabilized by a stabilizing group at the N-terminus, the C-terminus, or both. The stabilizing group can be a small stable protein, such as the Rop protein, glutathione sulfotransferase, thioredoxin, maltose binding protein, or glutathione reductase, an α-helical moiety, or one or more proline residues.
REFERENCES:
patent: 4711847 (1987-12-01), Konig et al.
patent: 4725535 (1988-02-01), Sonenshein et al.
patent: 4732864 (1988-03-01), Tolman
patent: 4987070 (1991-01-01), Magota et al.
patent: 5049545 (1991-09-01), Lobermann et al.
patent: 5093241 (1992-03-01), Bennett et al.
patent: 5175101 (1992-12-01), Gotz et al.
patent: 5187261 (1993-02-01), Latta et al.
patent: 5212083 (1993-05-01), Haldenwang
patent: 5270181 (1993-12-01), McCoy et al.
patent: 5292646 (1994-03-01), McCoy et al.
patent: 5302519 (1994-04-01), Blackwood et al.
patent: 5380712 (1995-01-01), Ballance et al.
patent: 5427927 (1995-06-01), Meyer et al.
patent: 5445954 (1995-08-01), Huang et al.
patent: 5589364 (1996-12-01), Williams et al.
patent: 5633229 (1997-05-01), Kokryakov et al.
patent: 5635182 (1997-06-01), McCoy et al.
patent: 5646016 (1997-07-01), McCoy et al.
patent: 5654451 (1997-08-01), Kari
patent: 5665863 (1997-09-01), Yeh
patent: 5677172 (1997-10-01), Makarow
patent: 5741646 (1998-04-01), Sherley et al.
patent: 5759802 (1998-06-01), Maki et al.
patent: 5766883 (1998-06-01), Ballance et al.
patent: 5792831 (1998-08-01), Maloy
patent: 5804553 (1998-09-01), Kokryakov et al.
patent: 5807746 (1998-09-01), Lin et al.
patent: 5888763 (1999-03-01), Hanafusa et al.
patent: 6057129 (2000-05-01), Young et al.
patent: 6143524 (2000-11-01), McCoy et al.
patent: 6165470 (2000-12-01), Becquart et al.
patent: 6180343 (2001-01-01), Anderson et al.
patent: 6329209 (2001-12-01), Wagner et al.
patent: 6548632 (2003-04-01), Anderson et al.
patent: 6562617 (2003-05-01), Anderson et al.
patent: 6566498 (2003-05-01), Ni, Jr. et al.
patent: 6630197 (2003-10-01), Wood et al.
patent: 6686179 (2004-02-01), Fleer et al.
patent: 6818611 (2004-11-01), Altman
patent: 7365162 (2008-04-01), Altman et al.
patent: 2001/0034333 (2001-10-01), Kosak
patent: 2001/0056075 (2001-12-01), Gyuris et al.
patent: 2003/0190740 (2003-10-01), Altman
patent: 2004/0235091 (2004-11-01), Altman
patent: 2007/0099262 (2007-05-01), Anderson et al.
patent: WO 90/07862 (1990-07-01), None
patent: WO 91/01743 (1991-02-01), None
patent: WO 92/07071 (1992-04-01), None
patent: WO 93/03156 (1993-02-01), None
patent: WO 93/23544 (1993-11-01), None
patent: WO 96/40721 (1996-12-01), None
patent: WO 97/04110 (1997-02-01), None
patent: WO 97/29127 (1997-08-01), None
patent: WO 98/22141 (1998-05-01), None
patent: WO 98/22141 (1999-01-01), None
patent: WO 99/35494 (1999-07-01), None
patent: WO 99/36554 (1999-07-01), None
patent: WO 99/53079 (1999-10-01), None
patent: WO 00/22112 (2000-04-01), None
patent: WO 01/45746 (2001-06-01), None
patent: WO 01/45746 (2001-10-01), None
patent: WO 01/77137 (2001-10-01), None
patent: WO 01/79258 (2001-10-01), None
patent: WO 01/79442 (2001-10-01), None
patent: WO 01/79443 (2001-10-01), None
patent: WO 01/79444 (2001-10-01), None
patent: WO 01/79480 (2001-10-01), None
patent: WO 01/79443 (2002-02-01), None
patent: WO 01/79444 (2002-05-01), None
patent: WO 01/79442 (2002-06-01), None
patent: WO 2004/011485 (2004-02-01), None
patent: WO 2004/011485 (2005-04-01), None
Tagami et al. (1995) Role of CRP in transcription activation atEscherichia colilac promoter: CRP is dispensable after the formation of open complex, Nucleic acids Res., 23, No. 4, pp. 599-605.
Saida et al. (2006) Expression of highly toxic genes inE. coli: special strategies and genetic tool, Curr. Protein Pept. Sci., vol. 7, No. 1, pp. 47-56.
Kentsis et al. (2004) Unfolded state of polyalanine is a segmented polyproline II helix, Proteins, vol. 55, No. 3, pp. 493-501.
Fasman (ed.),Prediction of Protein Structure and the Principles of Protein Conformation. Plenum Press, New York, NY; 1989. Title page, publisher's page, and table of contents only; 5 pages.
McTigue et al., “Crystal structures of a schistosomal drug and vaccine target: Glutathione S-transferase fromSchistosoma japanicaand its complex with the leading antischistosomal drug Praziquantel,” 1995J. Mol. Biol. 246:21-27.
Tang and Dill, “Native protein fluctuations: The conformational-motion temperature and the inverse correlation of protein flexibility with protein stability, ” 1998 J. Biomol. Struct. Dynam. 16(2):397-411.
“Structural Classification of Proteins” [online]. 1.73 release (Nov. 2007) Available on the Internet since 1994 [retrieved on Jan. 19, 2009]. Retrieved from //scop.mrc-lmb.cam.ac.uk/scop/; 3 pages.
Agerberth et al., “Amino Acid Sequence of PR-39, Isolation from Pig Intestine of a New Member of the Family of Proline-arginine-rich Antibacterial Peptides,” 1991.Eur J Biochem., 202(3):849-854.
Altman et al., “Characterization of Region in Mature LamB Protein That Interacts with a Component of the Export Machinery ofEscherichia coli”, 1990.J. Biol. Chem., 265(30):18148-18153.
Amann et al., “‘ATG vectors’ for regulated high-level expression of cloned genes inEscherichia coli”, 1985. Gene, 40:183-190.
Amann et al., “Tightly regulatedtacpromoter vectors useful for the expression of unfused and fused proteins inEscherichia coli”, 1988.Gene, 69-301-315.
Ast et al., “A rapid and sensitive bacterial assay to determine the inhibitory effect of ‘interface’ peptides on HIV-1 protease co-expressed inEscherichia coli,” 1998.J. Virological Methods, 71:77-85.
Bai et al., “Structural Specificity of Mucosal-Cell Transport and Metabolism of Peptide Drugs: Implication for Oral Peptide Drug Delivery”, 1992.Pharmaceutical Res., 9(8):969-978.
Bai et al., “Targeting of Peptide and Protein Drugs to Specific Sites in the Oral Route”, 1995.Crit. Rev. Ther. Drug Carrier Systems, 12(4):339-371.
Balbás et al., “Plasmid vector pBR322 and its special-purpose derivatives—a review”, 1986.Gene, 50:3-40.
Banner et al., “Structure of the ColE1 Rop Protein at 1.7 Å Resolution,” 1987.J. Mol. Biol., 196:657-675.
Barling et al., “Indirect [3H] Methyl Exchange as a General Method for Labeling Methionine Residues: Application to Calcitonin,” 1985.Analytical Biochemistry, 144(2):542-552.
Bechinger et al., “Structure and orientation of the antibiotic peptide magainin in membranes by solid-state nuclear magnetic resonance spectroscopy,” 1993.Protein Science, 2:2077-2084.
Bedarkar et al., “Polypeptide hormone-receptor interactions: the structure and receptor binding of insulin and glucagon,” 1978.Molecular Interactions and Activity in Proteins: Ciba Foundation Symposium, Excerpta Medica, Amsterdam, 60:105-121.
Beremand et al., “Synthesis, Cloning, and Expression inEscherichia coliof a Spinach Acyl Carrier Protein-1 Gene”, 1987.Arch. Biochem. Biophys., 256(1):90-100.
Betz et al., “De NovoDesign of Native Proteins: Characterization of Proteins Intended to Fold into Antiparallel, Rop-like, Four-Helix Bundles,” 1997.Biochemistry, 36:2450-2458.
Blanc et al., “Examination of the Requirement for an Amphiphilic Helical Structure in β-Endorphin through the Design, Synthesis, and Study of Model Peptides,”J. Biol. Chem., 258(13):8277-8284.
Bolivar et al., “Construction and Characterization of New Cloning Vehicles”, 1977.Gene, 2:95-113.
Brosius et al., “Regulation of ribosomal RNA promoters with a syntheticlacoperator,” 1984.Proc. Nat'l. Acad. Sci. USA, 81:6929-6933.
Desai Anand
Liu Samuel
Mueting Raasch & Gebhardt, P.A.
The University of Georgia Research Foundation, Inc
LandOfFree
Stabilized bioactive peptides and methods of identification,... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Stabilized bioactive peptides and methods of identification,..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Stabilized bioactive peptides and methods of identification,... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4274991