Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1989-09-13
1996-08-13
Moody, Patricia R.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435691, 4352523, 4353201, 435880, 435874, 935 42, 935 44, 935 45, 935 46, C12N 1568, C12N 1569, C12N 1574
Patent
active
055455417
ABSTRACT:
The stable maintenance of a replicon in a population of growing cells is ensured by providing the replicon with a sequence which encodes a product capable of killing the cell harboring the replicon or the progeny of the cell (or encodes a precursor for the product) and a sequence encoding an antagonist for the killing product (or a precursor for the antagonist). The antagonist is one which suppresses the killing product (or a precursor for the killing product) in cells harboring the replicon, whereas the antagonist activity decays when the replicon is lost from the cell so that the antagonist (or its precursor) is no longer continuously expressed. This means that the killing product (or its precursor) present in the now replicon-free cell is no longer suppressed by the antagonist, resulting in cell death.
Cells containing the thus stabilized replicon may be grown on a large scale without any significant loss of the replicon from the cell population even when no selection pressure is applied.
REFERENCES:
patent: 4760022 (1988-07-01), Molin et al.
patent: 4806471 (1989-02-01), Molin et al.
Ray et al. 1984. Plasmid 11: 272-275. (May 1984).
Chang et al. 1978. J of Bacti 134: 1141-1156.
Loh et al. 1986 (Nov.) FEMS Microbiology Letters 37: 179-182.
Loh et al. 1988. Gene 66: 259-268.
Gerdes et al. 1986. (Aug.) EMBO J. 5: 2023-2029.
Gerdes et al. 1988 J. Mol. Biol. 203: 119-130.
ATCC Bacteria Catalogue, 1985. #29055 on p. 72.
Ogura et al.: Proc. Natl. Acad. Sci. USA, 80, 1983, pp. 4784-4788.
Bech et al.: The EMBO Journal 4(4), 1985, pp. 1059-1066.
Gerdes et al.: J. Bacteriol. 161(1), 1985, pp. 292-298.
Prentki et al.: Gene 17, 1982, pp. 189-196.
Light et al.: The EMBO Journal 2(1), 1983, pp. 93-98.
Simmons and Kleckner: Cell 34, 1983, pp. 683-691.
Mizuno et al.: Proc. Natl. Acad. Sci. USA, 81, 1984, pp. 1966-1970.
Izant et al.: Cell 36, 1984, pp. 1007-1015.
Poulsen et al., "A family of genes encoding a cell-killing function may be conserved in all Gram-negative bacteria", Molecular Microbiology (1989) 3 (11), 1463-1472.
Light et al., "The Sites of Action of the two Copy Number Control Functions of Plasmid R1", Mol. Gen. Genet. (1982) 187 : 486, 493.
Bagdasarian et al., Gene. 16 (1981) 237-247.
Meacock, P. A.; Cohen, S. N. Partitioning of Bacterial Plasmids during cell division: a cis-acting locus that accomplishes stable plasmid inheritance, cell(20) 2 543-53 (1980).
Andersson Poul K.
Gerdes Kenn A.
Molin S.o slashed.ren
Rasmussen Poul B.
Benzon Pharma A/S
Cooper Iver P.
Moody Patricia R.
White B.
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