Stabilization of phosphoenol pyruvate carboxylase

Details Stabilization of phosphoenol pyruvate carboxylase Stabilization of phosphoenol pyruvate carboxylase

1974-12-20

1976-06-15

Naff, David M.

Textiles: fiber preparation

19599, 1951035R, 424 2, C07G 702, G01N 3114

Patent

active

039635783

ABSTRACT:
Phosphoenol pyruvate carboxylase is stabilized to have a shelf life of six months or more by incorporating the phosphoenal pyruvate carboxylase in an aqueous solution containing from 0.002 to 0.05 molar sodium phosphate, from 1 to 10, and preferably 3 to 8, m molar aspartate, from 0.02 to 0.1 m molar ethylene diamine tetraacetic acid, from 0.6 to 3.15, and preferably 0.8 to 1.2, molar ammonium sulfate and up to 20 volume percent glycerol. The sodium phosphate acts as a buffer to maintain the pH of the solution at from 6.0 to 6.8.

REFERENCES:
patent: 3296094 (1967-01-01), Cayle
patent: 3539450 (1970-11-01), Deutsch
patent: 3717550 (1973-02-01), Ziffer

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