Stabilization of oxidase enzyme-based test strips

Details Stabilization of oxidase enzyme-based test strips Stabilization of oxidase enzyme-based test strips

1992-02-25

1993-02-09

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving glucose or galactose

435 10, 435 11, 435 25, 435 28, C12Q 126, C12Q 154, C12Q 100, C12N 996

Patent

active

051852470

ABSTRACT:
A method and composition for the enzymatic assay of a liquid test sample, such as urine or serum, for a specific analyte, such as glucose. The method utilizes a dry phase test strip comprising a carrier matrix impregnated with a stabilized enzyme reagent composition including an oxidase enzyme, such as glucose oxidase, that is capable of interacting with the analyte of interest; a chromogen that changes color in response to the analyte-oxidase enzyme interaction to reveal the presence and/or concentration of the analyte in the test sample; and a stabilizer, such as a hydrazide, like GIRARD'S Reagent T, or an amino acid, like arginine, or an amino acid derivative, like the tris(hydroxymethyl)aminomethane sale of glutamic acid, to improve the heat stability and shelf life of the dry phase test strip.

REFERENCES:
patent: 4220713 (1980-09-01), Rittersdorf
Kunst et al. pp. 178-185 in Methods of Enzymatic Analysis VI, ed. Bergmeyer.

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