Stabilization of L-phenylalanine ammonia-lyase enzyme

Details Stabilization of L-phenylalanine ammonia-lyase enzyme Stabilization of L-phenylalanine ammonia-lyase enzyme

1983-09-06

1985-12-31

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing alpha or beta amino acid or substituted amino acid...

435116, 435128, 435188, 435232, C12P 1322, C12P 1306, C12P 1300, C12N 996, C12N 988

Patent

active

045621519

ABSTRACT:
A process for the synthesis of L-phenylalanines and analogues thereof from trans-cinnamate, ring substituted trans-cinnamates or various acrylate derivatives and ammonia or an ammonia donor in the presence of L-phenylalanine ammonia-lyase enzyme and a desensitizing agent such as polyhydric alcohols and polyethylene glycol-(400) is disclosed. The process embraces the discovery that compounds such as polyhydric alcohols and polyethylene glycol-(400) desensitize the L-phenyalanine ammonia-lyase enzyme to substantially higher substrate concentrations than practiced heretofore. In addition, polyhydric alcohols and polyethylene glycol-(400) substantially enhance the instantaneous rate of reaction and inhibit inactivation of the L-phenylalanine ammonia lyase enzyme over longer reaction periods.

REFERENCES:
patent: 4248704 (1981-02-01), Marconi et al.
Yamada et al. in Applied and Environmental Microbiology, vol. 42, No. 5, pp. 773-778 (1981).
Tanaka et al., J. Biochem., 81, pp. 963-970, (1977).
Hanson et al., Biochemistry, 18(8), pp. 1431-1438, (1979).
Birnbaum et al., Biotechnology Letters, 3 (8), pp. 393-400, (1981).
Douzou et al., Adv. in Protein Chemistry, 32, p. 79, (1978).
Yasumatsu et al., Agr. Biol. Chem. 29(7), pp. 665-671, (1954).
Ruis et al., Phytochemistry, 10, pp. 2627-2631, (1971).

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