Stabilization of intracellular enzymes

Details Stabilization of intracellular enzymes Stabilization of intracellular enzymes

1987-03-02

1990-08-21

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435180, 435182, 435188, 435234, C12P 1924, C12N 1108

Patent

active

049505963

ABSTRACT:
The subject invention concerns a process for stabilizing intact or ruptured microbial cells having glucose isomerase associated therewith. Specifically exemplified is a process for stabilizing glucose isomerase producing cells of a microorganism belonging to the genus Ampullariella. In the invention process the whole or ruptured microbial cells are contacted with a partially carboxyalkylated- or partially phosphonoalkylated-cationic polyelectrolyte, for example, a partially carboxymethylated polyethyleneimine to flocculate and stabilize the cells. The flocculated cells are further stabilized by encapsulation with a partially carboxyalkylated- or partially phosphonoalkylated-cationic polyelectrolyte. The encapsulation can be done prior to or after the flocculated cells are crosslinked. The net effect is manifested by a dramatic increase in the half-life of the glucose isomerase.

REFERENCES:
patent: 4355105 (1982-10-01), Lantero, Jr.
patent: 4675292 (1987-06-01), Houtchens et al.

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