Stabilization of glucose oxidase apoenzyme

Details Stabilization of glucose oxidase apoenzyme Stabilization of glucose oxidase apoenzyme

1981-04-17

1982-12-28

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 13, 435 20, 435 21, 435 22, 435 19, 435 25, 435 16, 435 28, 435190, 435188, 435805, C12N 904, C12N 996, C12Q 126, C12Q 140, C12Q 144, C12Q 146, C12Q 148, C12Q 156

Patent

active

043662439

ABSTRACT:
A stabilized composition comprising apoglucose oxidase is disclosed. In addition to the apoenzyme, a stabilizing agent comprising poly(vinyl alcohol), bovine serum albumin or mixtures thereof is included. The composition can also comprise, in addition to the apoenzyme and stabilizing agent, reagents for a homogeneous specific binding assay system capable of producing a detectable response which is a function of a ligand in, or the ligand binding capacity of a test sample, wherein the apoenzyme is itself one of the reagents in the assay system. The stabilized composition can be utilized in a test device for assaying a ligand in, or the ligand binding capacity of a liquid sample, whereby the device comprises a test device incorporated with the composition.

REFERENCES:
patent: 3413198 (1968-11-01), Deutsch
patent: 4238565 (1980-12-01), Hornby et al.
patent: 4286062 (1981-08-01), Stekolnikov
Swoboda, Biochim. Biophys. Acta, 175, 365-379 (1969).

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