Stabilization of coenzymes in aqueous solution

Details Stabilization of coenzymes in aqueous solution Stabilization of coenzymes in aqueous solution

1980-02-13

1983-07-19

Naff, David M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Stablizing an enzyme by forming a mixture, an adduct or a...

435 12, 435 17, 435 18, 435 26, C12N 996, C12Q 158, C12Q 150, C12Q 134

Patent

active

043944493

ABSTRACT:
A stabilized aqueous coenzyme solution is disclosed for use in the clinical assay of a selected biological constituent through the enzymic reaction of an enzyme with a selective substrate for the enzyme and a coenzyme. The aqueous coenzyme solution comprises at least one first enzyme and a first substrate for selectively reacting with the first enzyme in the assay, a coenzyme for interacting with the first enzyme and first substrate in the assay which coenzyme degrades forming a coenzyme conversion product, and at least one second enzyme and second substrate selective for the second enzyme which react with the coenzyme conversion product to regenerate the coenzyme.

REFERENCES:
patent: 3413198 (1968-11-01), Deutsch
patent: 4189536 (1980-02-01), Green
patent: 4218536 (1980-08-01), Maurukas
patent: 4247633 (1981-01-01), Case et al.
patent: 4277562 (1981-07-01), Modrovich

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