Stabilization of cholinesterase, detector kit using stabilized c

Details Stabilization of cholinesterase, detector kit using stabilized c Stabilization of cholinesterase, detector kit using stabilized c

1980-06-16

1982-04-13

Hruskoci, Peter A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435179, 435805, 435810, C12Q 146

Patent

active

043248586

ABSTRACT:
The stability of a cholinesterase (particularly a cholinesterase solution impregnated into a porous material and air-dried) can be improved by: (a) buffering the cholinesterase solution with a zwitterionic buffer, e.g. a buffer having a sulfonic acid group and a protonatable amine group, and, preferably, (b) further drying the impregnated, air-dried porous material under a high vacuum (e.g. 0.01 mm Hg or less) at normal ambient temperatures. The most useful porous materials are sheet-like in nature; that is, they have only two major surfaces. An impregnated, sheet-like material of this invention can be used in a cholinesterase inhibitor detector kit. A typical kit of this type provides a simple means for detecting, inter alia, environmental cholinesterase-inhibiting pollutant, e.g. organophosphorous pesticides and nerve agents.

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Makinen, et al., "Reactivity and Cryoenzymology of Enzymes in the Crystalline State," Ann. Rev. Biophys. Bioeng. 1977, pp. 301-315.

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