Stabilization of carrier-bound enzymes by treatment with a bifun

Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an...

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435174, C12N 1114, C12N 1100

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active

049406647

ABSTRACT:
The stability of granular carrier-bound enzymes is improved by further treating the carrier-bound enzyme with a bifunctional cross-linking agent and a polyamine. A particularly preferred cross-linking agent is glutaric dialdehyde, and a particularly preferred polyamine is polyethylene imine. Preferably, the cross-linking agent is used in an amount of 5 to 250 mg per 1 gram of carrier-bound enzyme and is dissolved in a 0.1 to 10% (W/V) aqueous solution, and the polyamine is used in an amount of 3 to 240 mg per 1 gram of dry carrier and is dissolved in a 0.1 to 20% (W/V) aqueous solution.

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