Stabilization of carboxyl esterase

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

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435141, 435196, 435197, 435188, C12P 740, C12P 752, C12N 918, C12N 996

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active

052388310

ABSTRACT:
Carboxyl esterase is inactivated by several chemical compounds such as naproxen or diclofop. By substituting or modifying certain basic residues of the carboxyl esterase, this enzyme shows improved stability properties during application. In this way it is possible to perform stereospecific hydrolysis reactions on industrial scale even at high substrate concentrations.

REFERENCES:
patent: 4101380 (1978-07-01), Rubinstein et al.
patent: 4886750 (1989-12-01), Bertala et al.
Royer et al., "Cross-Linking of Reversibly Immobilized Enzymes", FEBS Letters, 80:1, Aug. 1977, pp. 89-94.
Lombardo, D., "Catalytic Properties of Modified Human Pancreotic Carboxylic Ester Hydrolase", Biochemica et Biophysica Acta, 700(1), 75-80, 1982.

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