Stabilization of analytes

Details Stabilization of analytes Stabilization of analytes

1995-06-07

1997-08-26

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435188, 436 8, 436 16, 436 18, 436512, 436176, 436826, C12Q 170

Patent

active

056609787

ABSTRACT:
A method for stabilizing analytes with antibodies and antibody fragments comprises dissolving the analyte in a liquid to form a solution, adding analyte-specific antibodies, fragments of such antibodies, or both to the solution, heating the solution, and then cooling and filtering the solution. The filtered solution may be diluted in a suitable matrix.

REFERENCES:
patent: 4157280 (1979-06-01), Halbert et al.
patent: 4267272 (1981-05-01), Josephson
patent: 4652524 (1987-03-01), Modrovich
Sternberger et al., "The unlabeled antibody enzyme method of immunohistochemistry," J. Histochem. Cytochem. 18: 315-333 (1970).
Sawada et al., "Human prostatic acid phosphatase (EC-3.1.3.24) Stabilization of prostatic acid phosphatase against thermal inactivation by the homologous antibody," Chem Pharm Bull (Tokyo) 29:2935-2939 (1981), abstract only.
Cheridnickova et al., "Evidence for the stabilizing effect of antibodies on the subunit association of glyceraldehyde-3-phosphate dehydrogenase," Mol. Immunol. 18: 1055-1064 (1981), abstract only.
Melchers et al., "Enhanced stability against heat denaturation of E. coli wild type and mutant .beta.-galactosidase in the presence of specific antibodies," Biochem. Biophys. Research Comm. 40: 570-575 (1970).
Boyd et al., Fundamentals of Immunology, Third Edition (Interscience Publishers, Inc., New York) pp. 318-326 (1956).
Mason et al., "Preparation of peroxidase:Antiperoxidase (PAP) complexes for immunohistological labeling of monoclonal antibodies," J. Histochem. Cytochem. 30:1114-1122 (1982).

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