Stabilization and reduction of background fluorescence of hydrox

Details Stabilization and reduction of background fluorescence of hydrox Stabilization and reduction of background fluorescence of hydrox

1988-09-12

1994-11-08

Scheiner, Toni R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 18, 435 19, 435183, 435195, 435196, 436 8, G01N 3353, C12Q 144

Patent

active

053626273

ABSTRACT:
Hydroxy coumarin ester enzyme substrates can be stored in a strong base at pH above 11 to stabilize the substrate against premature hydrolysis of the ester bond and to reduce the revel of contaminating hydroxy coumarin. When diluted in buffer at pH below 11 shortly before use in an enzyme assay, the substrate solution provides low background fluorescence.

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