Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Glycoprotein – e.g. – mucins – proteoglycans – etc.
Patent
1992-04-03
1995-01-17
Draper, Garnette D.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Glycoprotein, e.g., mucins, proteoglycans, etc.
530324, 514 2, 514 8, 514 12, C07K 300, A61K 3702, A61K 3700, A01N 3718
Patent
active
053826582
ABSTRACT:
Herein described are variants of parathyroid hormone that retain significant PTH activity and are substantially resistant to trypsin and trypsin-like enzymes. The variants are useful pharmaceutically, to treat bone disorders such as osteoporosis and in other therapeutic applications. Specific embodiments of the invention include [His.sup.25 ]PTH and [His.sup.25 His.sup.26 Leu.sup.27 ]PTH.
REFERENCES:
patent: 4423037 (1983-12-01), Rosenblatt et al.
patent: 4698328 (1987-10-01), Neer et al.
patent: 4761406 (1988-08-01), Flora et al.
patent: 4968669 (1990-11-01), Rosenblatt et al.
Reppe et al, "Characterization of a K26Q Site-Directed Mutant Of Human Parathyroid Hormone Expressed In Yeast", J. Biol. Chem., 266:14198-14201, (1991).
Rosenblatt et al., "Modification Of The Arginines In Parathyroid Hormone: Effect On Biological Activity," Biochemistry, 17:3188, (1978).
Rodan et al, "Factors Associated With Humoral Hypercalcemia Of Malignancy Stimulate Adenylate Cyclase In Osteoblastic Cells", J. Clin. Invest., 72:1511-1515, (1983).
Rabbani et al, "Influence Of The Amino-Terminus On in Vitro and in Vitro Biological Activity Of Synthetic Parathyroid Hormone-Like Peptides Of Malignancy", 123:2709-2716, (1988).
Kimura et al, "Solution Synthesis Of [ASN.sup.76 ]-Human Parathyroid Hormone (1-84)", Biochem. Biophys. Res. Comm., 114:493-499 (1983).
Keutmann, "Current Research On Calcium Regulating Hormones", University of Texas Press, pp. 57-63, (1987).
Rosenblatt et al, "Chemical And Biological Properties Of Synthetic, Sulfur-free Analogues Of Parathyroid Hormone", J. Biol. Chem., 251:159-164, (1976).
Fairwell et al, "Total Solid-Phase Synthesis, Purification, and Characterization Of Human Parathyroid Hormone-(1-84)", Biochemistry, 22:2691, (1983).
Goud et al, "Solid-Phase Synthesis And Biologic Activity Of Human Parathyroid Hormone (1-84)", J. Bone Min. Res., 6(8):781-789, (1991).
Wosnick et al, "Total Chemical Synthesis And Expression in Escherichia coli Of A aize Glutathione-Transferase (GST) Gene", Gene, 76:153-160, (1989).
Barnett et al, "Rapid Generation Of DNA Fragments By PCR Amplification Of Crude, Synthetic Oligonucleotides", Nucl. Acids Res., 18(10):3094 (1990).
Hendy et al, "Nucleotide Sequence Of Cloned cDNAs Encoding Human Preproparathyroid Hormone", Proc. Natl. Acad. Sci. U.S.A., 78:7365-7369, (1981).
Kunkel T., "Rapid And Efficient Site-Specific Mutagenesis Without Phenotypic Selection", Proc. Natl. Acad. Sci. USA, 82:488-492, (1985).
Higuchi et al, "A General Method Of in vitro Preparation And Specific Mutagenesis Of DNA Fragments: Study Of Protein And DNA Interactions", Nucl. Acids Res., 16:7351-7367, (1988).
Sarkar et al, "The Megaprimer Method Of Site-Directed Mutagenesis", Biotechniques, 8(1):404-407, (1990).
Rabbani et al, "Synthesis And Characterization Of Extended And Deleted Recombinant Analogues Of Parathyroid Hormone-(1-84): Correlation Of Peptide Structure With Function", Biochemistry, 29:10080-10089.
Laemmli, "Cleavage Of Structural Proteins During The Assembly Of The Head Of Bacteriophage T4", Nature, 227:680-685, (1970).
Schagger et al, "Tricine-Sodium Dodecyl Sulfate-Polacrylamide Gel Electrophoresis For The Separation Of Proteins In The Range From 1 to 100 kDa", Analytical Biochemistry, 166:368-379, (1987).
Reeve et al.; Anabolic Effect of Human Parathyroid Hormone Fragment on a Trabecular Bone in Involutional Osteoporosis: A Multicentre Trial; British Medical Journal (7 Jun. 1980) pp. 1340-1344.
Mosekilde et al.; The Anabolic Effects of Human Parathyroid Hormone (hPTH) on Rat Vertebral Body Mass Are also Reflected in the Quality of Bone, Assessed by Biomechanical Testing: A Comparison Study between hPTH-(1-34) and hPTH-(1-84); The Endocrine Society (vol. 129, No. 1).
Bozzato Richard P.
Kronis K. Anne
Allelix Biopharmaceuticals Inc.
Draper Garnette D.
Glaxo Canada Inc.
Wang Gian P.
LandOfFree
Stability-enhanced variants of parathyroid hormone does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Stability-enhanced variants of parathyroid hormone, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Stability-enhanced variants of parathyroid hormone will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-747653