Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Insect cell – per se
Patent
1998-10-08
2000-08-15
Lankford, Jr., Leon B.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Insect cell, per se
435384, 435387, C12N 500
Patent
active
061035262
ABSTRACT:
Disclosed and claimed is a new insect cell line, Sf900+, ATCC CRL-12579. The insect cell line was established from Lepidoptera, Noctuidae, Spodoptera frugiperda Sf-9 (ATCC CRL-1771) through multiple rounds of limiting dilution and selection in a serum-free insect medium supplemented with added human insulin. The insect cell line is useful in BEVS or as an adjuvant and has many characteristics and advantages. Also disclosed and claimed are recombinant proteins from recombinant baculovirus expression in insect cells such as Sf900+ cells, for instance, HA, NA, EPO, CD4, CEA, and thrombospondin.
REFERENCES:
Hink and Butz. In Vitro Cellular & Developmental Biology. 21(6). pp. 333-339, Jun. 1985.
DeBartolomeis James
Foellmer Harald G.
Knell John
Smith Gale E.
Voznesensky Andrei I.
Kowalski Thomas J.
Lankford , Jr. Leon B.
Protein Sciences Corporation
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