Split ubiquitin protein sensor

Details Split ubiquitin protein sensor Split ubiquitin protein sensor

1994-04-22

1996-04-02

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

530350, 530403, 435 697, 4351723, 4353201, 536 234, 935 10, 935 76, 935 79, 935 80, C07K 1900, C12Q 168, C12N 1562, C12N 1563

Patent

active

055039774

ABSTRACT:
Disclosed are novel compositions and methods useful for studying interactions between proteins. An N-terminal subdomain and a C-terminal subdomain of ubiquitin are linked to a pair of proteins or peptides to be examined for their ability to interact. When contacted with one another, a quasi-native ubiquitin moiety is reconstituted provided that the protein or peptide pair do, in fact, interact (bind) with one another. The quasi-native ubiquitin moiety is recognized and cleaved by ubiquitin-specific proteases after the last residue of ubiquitin. The cleavage at the quasi-native ubiquitin moiety within a linear protein fusion is the indication of interaction between the protein or peptide pair.

REFERENCES:
patent: 5283173 (1994-02-01), Fields et al.
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Fields and Song (1989). A novel genetic system to detect protein-protein interactions. Nature 340, 245-246.
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Stockman et al. (1993). Heternuclear three-dimensional NMR spectroscopy of a partially denatured protein: The A-state of human ubiquitin. J. Biomolecular NMR 3, 285-296.

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