Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1987-04-15
1989-12-12
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
435886, 4351723, 435183, 435235, 43525233, 43525235, 435320, 435849, 435872, 536 27, 935 9, 935 24, 935 60, 935 72, C12N 1500, C12N 120
Patent
active
048867578
ABSTRACT:
A novel gene conferring resistance to spiramycin in Streptomyces and related organisms was cloned from a genomic library of Streptomyces ambofaciens DNA. A thirty-one Kb fragment of S. ambofaciens DNA including the spiramycin-resistance gene was isolated from this library on a cosmid designated pKC592. The novel spiramycin-resistance gene can be isolated on an .about.2.9 Kb BamHI fragment by subcloning restriction fragments obtained from the pKC592 insert DNA. This BamHI fragment contains all of the information required for the expression of the spiramycin resistant phenotype in Streptomyces. Vectors and transformants containing the novel spiramycin resistance gene are provided.
REFERENCES:
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Eli Lilly and Company
Norman Douglas K.
Peet Richard C.
Whitaker Leroy
Wiseman Thomas G.
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