Spin filter for removing substantially cell-free culture medium

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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435286, 435311, 435312, 435813, 435284, C12M 302, C12M 306

Patent

active

050195123

ABSTRACT:
A spin filter for removing substantially cell-free medium from a stirred suspension culture vessel, wherein a stationary baffle is arranged in the interior space of the spin filter to disrupt liquid flow therein and minimize the tendency of cells to collect on and foul the inner-facing filter surfaces, the filter surface being sized to substantially exclude materials greater than about 8 to 10 microns, and the spin filter being rotatable independent of, and in a rotational direction opposite to, the vessel stirring device.

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patent: 4586779 (1986-06-01), Ono
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patent: 4649118 (1987-03-01), Anderson
Perry's Chemical Engineer's Handbook, 6th Edition, pp. 27-5-27-6.
Varecka, R. et al., "Use of a Rotating Wire Cage for Retention of Animal Cells in a Perfusion Fermentor", Develop. Biol. Standard., vol. 66, pp. 269-272 (1987).
"Continuous Cultures Without Wash-Out", Biotechnology, Nov. 1984.
Feder, Joseph et al., "Mass Culture of Mammalian Cells in Perfusion Systems", ABL, pp. 24-36, Jan.-Feb., 1985.
"ViTis-BioSpin Dynamic Filter", Product Literature.

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