Spillover collection of sparse components such as mononuclear ce

Details Spillover collection of sparse components such as mononuclear ce Spillover collection of sparse components such as mononuclear ce

1995-04-14

1998-01-06

Cooley, Charles E.

Imperforate bowl: centrifugal separators

Process

494 45, B04B 1104

Patent

active

057048894

ABSTRACT:
A centrifuge apparatus is used for collecting white blood cells (WBC), primarily mononuclear cells, from whole blood stratified into layers. A thin mononuclear (MNC) layer is formed at the interface of red blood cells and plasma. A barrier is positioned in the separation vessel of the centrifuge at a location to intercept the thin layer. MNC fluid is allowed to pool behind the barrier before collection is started. To collect the MNC pool, the stratified red blood cell layer is raised from below the interface level by slowing or reversing flow in the RBC exit line thereby causing the MNC pool to spill over the barrier into a well in which a collect line is positioned. Collection ceases when a desired percentage of the pool is removed and the normal position of the interface is re-established; thereafter the pool builds again. By raising the MNC pool from below, improvements in purity and collect volume are achieved. The collection procedure can be useful for harvesting granulocytes and, in general, any sparse stratified component of a centrifuged solution where the sparse component is layered between more dense and less dense strata.

REFERENCES:
patent: 1723212 (1929-08-01), Sheeran
patent: 3452924 (1969-07-01), Schultz
patent: 3655123 (1972-04-01), Judson et al.
patent: 3708110 (1973-01-01), Unger et al.
patent: 3737096 (1973-06-01), Jones et al.
patent: 3858795 (1975-01-01), Joyce
patent: 3858796 (1975-01-01), Unger et al.
patent: 4007871 (1977-02-01), Jones et al.
patent: 4010894 (1977-03-01), Kellogg et al.
patent: 4091989 (1978-05-01), Schultz
patent: 4094461 (1978-06-01), Kellogg et al.
patent: 4120448 (1978-10-01), Cullis
patent: 4151844 (1979-05-01), Cullis et al.
patent: 4187979 (1980-02-01), Cullis et al.
patent: 4316576 (1982-02-01), Cullis et al.
patent: 4330080 (1982-05-01), Mathieu
patent: 4356958 (1982-11-01), Kolobow et al.
patent: 4386730 (1983-06-01), Mulzet
patent: 4387848 (1983-06-01), Kellogg et al.
patent: 4430072 (1984-02-01), Kellogg et al.
patent: 4447221 (1984-05-01), Mulzet
patent: 4531932 (1985-07-01), Luppi et al.
patent: 4557719 (1985-12-01), Neumann et al.
patent: 4636193 (1987-01-01), Cullis
patent: 4647279 (1987-03-01), Mulzet et al.
patent: 4668214 (1987-05-01), Reeder
patent: 4708712 (1987-11-01), Mulzet
patent: 4807676 (1989-02-01), Cerny et al.
patent: 4838852 (1989-06-01), Edelson et al.
patent: 4850995 (1989-07-01), Tie et al.
patent: 4934995 (1990-06-01), Cullis
patent: 4940543 (1990-07-01), Brown et al.
patent: 5006103 (1991-04-01), Bacehowski et al.
patent: 5104526 (1992-04-01), Brown et al.
patent: 5141486 (1992-08-01), Antwiler
patent: 5224921 (1993-07-01), Dennehey et al.
patent: 5260598 (1993-11-01), Brass et al.
patent: 5281342 (1994-01-01), Biesel et al.
patent: 5322620 (1994-06-01), Brown et al.
A.L. Jones, "Blood Cell Washing," IBM Technical Disclosure Bulletin, vol. 10 No. 7, Dec. 1967, pp. 944-945.
Fresenius MT AS 104 blood cell separator, Apr. 6, 1990(OP), Operating Instructions, Chapter 2.
Gebrauchsanweisung, Kapitel 2, Fresenius MT Blutzellseparator AS 104, Jul. 3, 1992(GA); English translation Part 12.3.7.9, "Cycle Control and Spillover Parameters," Software version 4.6.
Operator's Manual, 7-19-3-185, Fenwal.RTM. CS-3000.RTM. Plus Blood Cell Separator, Oct. 1990.
Owner's Operating and Maintenance Manual, Haemonetics Mobile Collection System, Dec. 1, 1991, Rev.B., Part No. 35349, Haemonetics Corporation, Braintree, MA 02184.

Affiliated with

Also associated with

Rating

Spillover collection of sparse components such as mononuclear ce does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Spillover collection of sparse components such as mononuclear ce, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Spillover collection of sparse components such as mononuclear ce will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2324556