Sphingosine-1-phosphate essentially free of L-threo isomer

Organic compounds -- part of the class 532-570 series – Organic compounds – Phosphorus esters

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C07F 909

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056634048

ABSTRACT:
A method of inhibiting tumor cell chemotactic and/or chemoinvasion motility comprising contacting the tumor cell with an inhibitory amount of an agent selected from the group consisting of sphingosine-1-phosphate, derivatives of sphingosine-1-phosphate and mimetics of the sphingosine-1-phosphate or of the derivatives. A method of inhibiting phagokinetic activity of tumor cells and neutrophils comprising contacting the cells with a phagokinetic inhibitory amount of an agent selected from the group consisting of sphingosine-1-phosphate, derivatives of sphingosine-1-phosphate, and mimetics of the sphingosine-1-phosphate or of the derivatives. A method of inhibiting tumor cell metastasis comprising administering to a host in need of treatment a metastasis inhibitory amount of an agent selected from the group consisting of sphingosine-1-phosphate, derivatives of sphingosine-1-phosphate, and mimetics of the sphingosine-1-phosphate or of the derivatives, and pharmaceutically acceptable salts of the agent. A method of inhibiting inflammation due to motility and invasion into blood vessel walls of neutrophils comprising administering to a host in need of treatment an inflammation inhibitory amount of an agent selected from the group consisting of sphingosine-1-phosphate, derivatives of sphingosine-1-phosphate, and mimetics of the sphingosine-1-phosphate or of the derivatives, and pharmaceutically acceptable salts of the agent. A method of preparing sphingosine-1-phosphate and its derivatives.

REFERENCES:
patent: 5110987 (1992-05-01), Liotta et al.
"Enzymatic Quantification of Sphingosine in the Picomole Range in Cultured Cells", Van Veldhoven, et al., Analytical Biochemistry 183, 177-189 (1989).
Chemical Abstracts 92 (5), No. 41295 (1980).
A.S. Bushnev et al., Bioorg. Khim. 5 (9), 1381-1384 (1979).
Stoffel, W. et al. Hoppe-Seyler's Z. Physiol. Chem. 351, 635-642 (1970).
Van Veldhoven, P.P. et al. Journal of Lipid Research, vol. 30, pp. 611-616 (1989).

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