Sphingomyelin detecting probe

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S235100, C435S320100

Reexamination Certificate

active

11223974

ABSTRACT:
Proteins useful as sphingomyelin detecting probes, which specifically recognize sphingomyelin and have low cytotoxicity. The proteins of the present invention may be obtained by deletion of between about 110 to 170 amino acid residues from the N terminal or C terminal of earthworm toxin Lysenin 1. Kits for detecting sphingomyelin are also disclosed.

REFERENCES:
patent: 4686191 (1987-08-01), Itoh et al.
patent: 4939094 (1990-07-01), Kuga et al.
patent: 5160735 (1992-11-01), Yasumura et al.
patent: 6569464 (2003-05-01), Mukherjee et al.
patent: 58110600 (1983-07-01), None
patent: 2-227075 (1990-09-01), None
patent: 3-22979 (1991-01-01), None
E. Kiyokawa et al. Biochemistry (2004) 43, 9766-9773.
S. Lange, et al. J. Biol. Chem. (1997) 272(33), pp. 20884-20892.
C. Alvarez, et al., Toxicon (1998) 36(1), pp. 165-178.
C.A. Valcarcel, et al. Biophysical Journal (2001) 80, pp. 2761-2774.
English Language Abstract of JP 58-110600.
Nishi et al., Agric. Biol. Chem., vol. 48, No. 3. pp. 669-6750 (1984).
Miyaji et al., Agric. Biol. Chem., vol. 53, No. 1, pp. 277-279 (1989).
Sekine et al., Proc. Natl. Acad. Sci., vol. 82, pp. 4306-4310 (1985).
Yanisch-Perron et al., Gene, vol. 33, pp. 103-119 (1985).
English Language Abstract of JP 3-22979.
Miyaji et al., Cytotechnology, vol. 3, pp. 133-140 (1990).
English Language Abstract of JP 2-227075.
Seed, B., Nature, vol. 329, pp. 840-842 (1987).
Mizukami et al., J. Biochem. vol. 101, pp. 1307-1310 (1987).
Nishibayashi et al., Plant Cell Reports, vol. 15, pp. 809-814 (1996).
Jefferson et al., The EMBO J., vol. 6, No. 13, pp. 3901-3907 (1987).
Terada et al., Mol. Gen. Genet., vol. 220, pp. 389-392 (1990).
Sekizawa et al., Gene, vol. 191, pp. 97-102 (1997).
Toshihide Kobayashi et al., “Lysenin: Sphingomyelin Specific Probe”, Molecular Biology of the Cell, vol. 11, pp. 314A (2000).
Yoshiyuki Sekizawa et al., “Molecular Cloning of cDNA for Lysenin, A Novel Protein in the EarthwormEisenia foetidathat Causes Contraction of Rat Vascular Smooth Muscle”, Gene, vol. 191, pp. 97-102 (1997).
Akiko Yamaji et al., “Lysenin, A Novel Sphingomyelin-Specific Binding Protein”, The Journal of Biological Chemistry, vol. 273, No. 9, pp. 5300-5306 (1998).
K. Itoh et al., “Lysenin-Sphingomyelin Bindingon Cell Surface Increases During Oligodendrocyte Lineage In Vitro”, Society for Neuroscience Abstracts, vol 26, No. 1-2; pp. 601.3 (2000).
Masao Iwamori et al., “Sensitive Method for the Determination of Pulmonary Surfactant Phospholipid/Sphingomyelin Ratio in Human Amniotic Fluids for the Diagnosis of Respiratory Distress Syndrome by thin-Layer Chromatography-Immunostaining”, Analytical Biochemistry, vol. 238, pp. 29-33 (1996).
José A. Encinar et al., “Enzymatic Determination of Phosphatidylcholine, Sphingomyelin and Phosphatidylglycerol in Lipid Dispersions, Blood Cell Membranes and Rat Pulmonary Surfactant1)”, Eur. J. Chem Clin Biochem, vol. 34, pp. 9-15 (1996).
Maguy Lassegues et al., “Sequence and Expression of anEisenia-fetida-derived cDNA Clone that Encodes the 40-kDa Fetidin Antibacterial Protein”, Eur. J. Biochem., vol. 246, pp. 756-762 (1997).
SIGMA. Designing Custom Peptides. http://www.sigma-genosys.com/peptide—design.asp (Accessed Dec. 16, 2004. 2 pages.
HJC Berendsen. A. Glimpse of the Holy Grail? Science (1998) 282.642-643.
D Voet and JG Voet. Biochemistry, 2ndEdition. (1995). 235-241.
J. Rudinger. Characteristic of the amino acids as components of a peptide hormone sequence. In Peptide Hormones, JA Parsons, Ed. (1976) University Press, Baltimore. 2-7.
H. Kobayashi et al., Lethal and non-lethal responses if spermatozoa from a wide variety of vertebrates and invertebrates to lysenin, a protein from the coelomic fluid of the earthwormEisenia foetida. J. Exp. Zool. (2000) 286, 538-549.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Sphingomyelin detecting probe does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Sphingomyelin detecting probe, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Sphingomyelin detecting probe will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3771380

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.