Sphingolipid ceramide N-deacylase, methods for producing...

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Reexamination Certificate

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C536S023200, C536S063000, C536S024320, C536S024330, C435S320100, C435S252300, C435S325000

Reexamination Certificate

active

07364787

ABSTRACT:
The present invention relates to a novel sphingolipid ceramide N-deacylase (SCDase) having a wide substrate specificity; a method for enzymatically producing a lysosphingolipid or a sphingolipid derivative using the SCDase which is useful in the fields of medicine, carbohydrate engineering, cell engineering, and the like; the lysosphingolipid or sphingolipid derivative obtained by this production method; a gene which encodes a polypeptide having an SCDase activity useful in sphingolipid technology; a method for industrially producing a polypeptide having an SCDase deacylase activity and a recombinant polypeptide thereof using a transformant to which the gene is introduced; a probe or primer which hybridizes to the gene; and an antibody or a fragment thereof which specifically binds to the polypeptide.

REFERENCES:
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Office Action from related Chinese Patent Application with English language translation.
Izumi, et al., “A Novel Glycosphingolipid deacylase hydrolyzing globoside yields lysogloboside and fatty-acid”Glycoconjugate Journal, Aug. 1993, vol. 10, No. 4, p. 229.
M. Furusato, et al. “Molecular Cloning and Characterization of Sphingolipid Ceramide N-Deacylase From a Marine Bacterium, Shewanella alga G8”.J. Biol. Chem. 277(19): 17300-17307. (May 2002).

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