Spectroscopic method and apparatus for optically detecting abnor

Surgery – Diagnostic testing – Detecting nuclear – electromagnetic – or ultrasonic radiation

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600473, 356301, A61B 600

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active

060959827

ABSTRACT:
A method and apparatus for detecting tissue abnormality, particularly precancerous cervical tissue, through fluorescence or Raman spectroscopy, or a combination of fluorescence and Raman spectroscopy. In vivo fluorescence measurements were followed by in vitro NIR Raman measurements on human cervical biopsies. Fluorescence spectra collected at 337, 380 and 460 nm excitation were used to develop a diagnostic method to differentiate between normal and dysplastic tissues. Using a fluorescence diagnostic method, a sensitivity and specificity of 80% and 67% were observed for differentiating squamous intraepithelial lesions (SILs) from all other tissues. In accordance with another aspect of the invention, using Raman scattering peaks observed at selected wavenumbers, SILs were separated from other tissues with a sensitivity and specificity of 88% and 100%. In addition, inflammation and metaplasia samples are correctly separated from the SILs.

REFERENCES:
patent: 4479499 (1984-10-01), Alfano et al.
patent: 4556057 (1985-12-01), Hiruma et al.
patent: 4648892 (1987-03-01), Kittrell et al.
patent: 4675529 (1987-06-01), Kushida
patent: 4755684 (1988-07-01), Leiner et al.
patent: 4786813 (1988-11-01), Svanberg et al.
patent: 4798463 (1989-01-01), Koshi
patent: 4832483 (1989-05-01), Verma
patent: 4913142 (1990-04-01), Kittrell et al.
patent: 4930516 (1990-06-01), Alfano et al.
patent: 4967745 (1990-11-01), Hayes et al.
patent: 4973848 (1990-11-01), Kolobanov et al.
patent: 5003977 (1991-04-01), Suzuki et al.
patent: 5009655 (1991-04-01), Daignault, Jr. et al.
patent: 5014707 (1991-05-01), Schwarz et al.
patent: 5026368 (1991-06-01), Adair
patent: 5034010 (1991-07-01), Kittrell et al.
patent: 5036853 (1991-08-01), Jeffcoat et al.
patent: 5038039 (1991-08-01), Wong et al.
patent: 5042494 (1991-08-01), Alfano et al.
patent: 5046501 (1991-09-01), Crilly
patent: 5062431 (1991-11-01), Potter
patent: 5092331 (1992-03-01), Nakamura et al.
patent: 5104392 (1992-04-01), Kittrell et al.
patent: 5106387 (1992-04-01), Kittrell et al.
patent: 5115137 (1992-05-01), Andersson-Engels et al.
patent: 5125404 (1992-06-01), Kittrell
patent: 5131398 (1992-07-01), Alfano et al.
patent: 5168162 (1992-12-01), Oong et al.
patent: 5174297 (1992-12-01), Dalkuzono
patent: 5192278 (1993-03-01), Hayes et al.
patent: 5199431 (1993-04-01), Kittrell et al.
patent: 5201318 (1993-04-01), Rava et al.
patent: 5251613 (1993-10-01), Adair
patent: 5261410 (1993-11-01), Alfano et al.
patent: 5280788 (1994-01-01), Janes et al.
patent: 5290275 (1994-03-01), Kittrell et al.
patent: 5293872 (1994-03-01), Alfano et al.
patent: 5303026 (1994-04-01), Strobl et al.
patent: 5304173 (1994-04-01), Kittrell et al.
patent: 5318023 (1994-06-01), Vari et al.
patent: 5318024 (1994-06-01), Kittrell et al.
patent: 5337745 (1994-08-01), Benaron
patent: 5345941 (1994-09-01), Rava et al.
patent: 5348003 (1994-09-01), Caro
patent: 5348018 (1994-09-01), Alfano et al.
patent: 5369496 (1994-11-01), Alfano et al.
patent: 5377676 (1995-01-01), Vari et al.
patent: 5386827 (1995-02-01), Chance et al.
patent: 5408996 (1995-04-01), Salb
patent: 5413108 (1995-05-01), Alfano
patent: 5419323 (1995-05-01), Kittrell et al.
patent: 5421337 (1995-06-01), Richards-Kortum et al.
patent: 5421339 (1995-06-01), Ramanujam et al.
patent: 5421346 (1995-06-01), Sanyal
patent: 5450857 (1995-09-01), Garfield et al.
patent: 5452723 (1995-09-01), Wu et al.
patent: 5467767 (1995-11-01), Alfano et al.
patent: 5498875 (1996-03-01), Obremski et al.
patent: 5590660 (1997-01-01), MacAulay et al.
patent: 5596992 (1997-01-01), Haaland et al.
K. Schomacker et al., "Ultraviolet Laser-Induced Fluorescence of Colonic Tissue: Basic Biology and Diagnostic Potential," Lasers in Surgery and Medicine, 12:63-78, 1992.
R. Cothren et al., "Gastrointestinal Tissue Diagnosis by Laser-Induced Fluorescence Spectroscopy at Endoscopy," Gastrointestinal Endoscopy, 36:105-111, 1990.
S. Andersson-Engels et al., "Fluorescence Imaging and Points Measurements of Tissue: Appliations to the Demarcation of Malignant Tumors and Atherosclerotic Lesions from Normal Tissue," Photochemistry and Photobiology, 53:807-814, 1991.
R. Rava et al., "Early Detection of Dysplasia in Colon and Bladder Tissue Using Laser-Induced Fluorescence," SPIE, Optical Methods for Tumor Treatment and Early Diagnosis: Mechanisms and Techniques, 1426:68-78, 1991.
R. Cothren et al., "Argon Ion Laser-Induced Tissue Fluorescence: Clinical Spectroscopic Studies," SPIE, Optical Fibers in Medicine III, vol. 906, Abstract only, 1988.
S. Lam et al., "Detection of Dysplasia and Carcinoma In Situ by Ratio Fluorometry," Am. Rev. Respir. Dis., 146:1458-1461, 1992.
R.R. Alfano et al., "Optical Spectroscopic Diagnosis of Cancer in Normal Breast Tissues," J. Opt. Soc. Am. B., 6:1015-1023, May 1989.
R.R. Alfano et al., "Fluorescence Spectra from Cancerous and Normal Human Breast and Lung Tissues," IEEE Journal of Quantum Electronics, QE-23:1806-1811, Oct. 1987.
J. Hung et al., "Autofluorescenceof Normal and Malignant Bronchial Tissue," Lasers in Surgery and Medicine, 11:99-105, 1991.
R. Richards-Kortum et al., "Spectroscopic Diagnosis of Colonic Dysplasia," Photochemistry and Photobiology, 53:777-786, 1991.
C. Kapadia et al., "Laser-Induced Spectroscopy of Human Colonic Mucosa: Detection of Adenomatous Transformation," Gastroenterology, 99:150-157, 1990.
P. Wong et al., "Infrared Spectroscopy of Exfoliated Human Cervical Cells: Evidence of Extensive Structural Changes During Carcinogensis," Proc. Natl. Acad. Sci. USA, 88:10988-10992, 1991.
W. Glassman, "Ultraviolet Excited Fluorescent Spectra from Non-Malignant and Malignant Tissues of the Gynecological Tract," Lasers in the Lifesciences, 49-58, 1992.
W. Lohmann et al., "Native Fluorescence of the Cervix Uteri as a Marker for Dysplasia and Invasive Carcinoma," European Journal of Obstetrics & Gynecology and Reproductive Biology, 31:249-253, 1989.
N. Ramanujam et al., "Diagnosis of Cervical Intraepithelial Neoplasia Using Laser-Induced Fluorescence," Poster Presentation at Future Directions of Lasers in Surgery and Medicine III, 1993.
R. Walpole and R. Myers, Tests of Hypotheses, In: Probability and Statistics for Engineers and Scientists, 2nd Ed., Chapter 7:v, 238-259, 1978.
W. Dillon and M. Goldstein, Principal Components Analysis, In: Multivariate Analysis, Methods and Applications, Chapter 2:23-392, 1984.
C. Liu et al., "Raman, Fluorescence, and Time-Resolved Light Scattering as Optical Diagnostic Techniques to Separate Diseased and Normal Biomedical Media," J. Photochem. Photobiol. B: Biol., 16:187-209, 1992.
D. Redd et al., "Raman Spectroscopic Characterization of Human Breast Tissues: Implications for Breast Cancer Diagnosis," Applied Spectroscopy, 47(6):787-791, 1993.
R. Alfano et al., "Human Breast Tissues Studied by IR Fourier Transform Raman Spectroscopy," Lasers in the Life Sciences, 4(1):23-28, 1991.
G. Small et al., "Strategies for Coupling Digital Filtering with Partial Least-Squares Regression: Application to the Determinationof Glucose in Plasma by Fourier Transform Near-Infrared Spectroscopy," Anal. Chem., 65:3279-3289, 1993.
International Search Report dated Jul. 17, 1996 (TUUT:003P).
International Search Report dated Jul. 12, 1996 (TUUT:004P).
International Search Report dated Jul. 12, 1996 (TUUT:005P).
Alfano et al., "Laser Induced Fluorescence Spectroscopy from Native Cancerous and Normal Tissue," IEEE Journal of Quantum Electronics, QE-20(12):1507-1511, 1984.
Avrillier et al., "XeC1 Excimer Laser-Induced Autofluorescence Spectroscopy for Human Cerebral Tumours Diagnosis: Preliminary Study," SPIE, 1894:177-186, 1993.
Bergeron et al., "Complete Fluorescence Spectrum of a Normal and Atherosclerotic Aorta," Can. J. Phys., 66:1035-1039, 1988.
Bosshart et al., "Fluorescence Spectroscopy for Identification of Atherosclerotic Tissue," Cardiovascular Research, 26:620-625, 1992.
Bottiroli et al., "Natural Fluorescence of Normal and Neoplastic Human Colon: A Comprehensive "ex vivo" Study," Lasers in Surgery & Medicine, 16(1):48-60, 1995.
Clarke et al., "Spectroscopic Characterization of Cardiovascu

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