Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-07-25
1998-05-05
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
424941, 356328, 435 911, C12Q 168, C12P 1934
Patent
active
057472475
ABSTRACT:
The invention provides spectroscopic methods for detecting helicase activity and inhibitors of helicase activity. Samples are assayed for helicase activity by: (a) incubating a mixture of the sample, double-stranded nucleic acid and a suitable luminescent marker which lumineses selectively in the presence of double-stranded nucleic acid; (b) exposing the mixture to light capable of inducing luminescence from the marker; and (c) detecting the intensity of luminescence from the mixture. Alternatively, samples are assayed for helicase inhibitors by further including in the mixture a helicase and incubating the mixture under conditions whereby, but for the presence of an inhibitor of the helicase in the sample, the helicase would be capable of converting a portion of the double-stranded nucleic acid into single-stranded nucleic acid. In both assays, helicase activity is inversely proportional to the detected luminescense. The methods are particularly suited to high-throughput drug screening.
REFERENCES:
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Eggleston Angela K.
Kowalczykowski Stephen C.
Fredman Jeffrey
Jones W. Gary
Osman Richard Aron
The Regents of the University of California
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