Spectrophotometric quantitation of images in X-ray film and elec

Radiation imagery chemistry: process – composition – or product th – Including control feature responsive to a test or measurement

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430347, 436 2, 436 8, 436 80, 436171, 436175, 356326, 356319, 356432, 356443, G01N 2177, G01J 342

Patent

active

055716431

ABSTRACT:
Images comprised of silver such as X-rays and electrophoresis gel bands, wherein the size and darkness of the image provides useful information, are quantitated utilizing a spectrophotometer to quantify the silver which comprises the image. An image is isolated from X-ray film or an electrophoresis gel followed by removal therefrom of any silver which is present. The removed silver is complexed to cause an optical change. A spectrophotomer is then used to measure the absorbance and the absorbance measurement is compared with a calibrated reference standard.

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patent: 4876068 (1989-10-01), Castaneda
patent: 4920057 (1990-04-01), Castaneda
D. H. O. John & G. T. J. Field, A Textbook of Photographic Chemistry, Chapman & Hall Ltd., London, 6-7, (1963).
Holmquist, B., Methods Enzymol, 158, 6-14, (1988).
Molecular Dynamics brochures on densitometry instrumentation.
Pharmacia LKB Biotechnology brochure on the ImageMaster.TM. Electrophoresis Evaluation System.

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