Specific DNA probes for the identification of the Taenia solium

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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536 231, 536 2432, C12Q 168, C07H 2102, C07H 2104

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active

061565059

ABSTRACT:
The invention features compositions and methods for the detection of and differentiation between Taenia solium and/or Taenia saginata. Specifically, the invention features nucleic acid probes comprising specific repetitive sequences of T. solium and T. saginata. These sequences make it possible to distinguish, with a high level of sensitivity and specificity, the eggs of these species of Taenia, thus enabling the diagnosis of taeniasis and the identification of carriers of Taenia solium. The invention is of great importance in controlling spread of T. solium due to ingestion of infected pork and/or exposure to human carriers of T. solium, thus facilitating the eradication of human and swine cysticercosis. The invention is thus of great importance in supporting eradication of human and swine cysticercosis.

REFERENCES:
patent: 4740456 (1988-04-01), Kuhn et al.
Flisser, A. et al. Specific detection of Taenia saginata eggs by DNA hybridisation. Lancet 2:1429-1430, Dec. 1988.
Ahern, H. Biochemical, reagent kits offer scientists good return on investment. The Scientist 9(15):20, Jul. 1995.
Allan, J.C., et al., 1990, "Immunodiagnosis of Taeniasis by Coproantigen Detection," Parasitology 101: 473-7.
Allan, J.C., et al., 1992, Coproantigen detection for immunodiagnosis of echinococcosis and taeniasis in dogs and humans, Parasitology 104: 347-55.
Allan, J.C., et al., 1993, "Dipstick ELISA for the Detection of Taenia Coproantigens in Humans," Parasitology 107:79-85.
Bowles, J., et al., 1992, "Genetic Variants Within the Genus Echinococcus Identified by Mitochondrial DNA Sequencing," Mol. Biochem. Parasitol. 54:165-75.
Flisser, A., 1990, "New Approaches in the Diagnosis of Taenia solium cysticercosis and Taeniasis," Ann. Parasitol. Hum. Comp. 65:95-98.
Garey, J.R., and Wolstenholme, D.R., 1989, "Platyhelminth Mitochondrial DNA: Evidence for Early Evolutionary Origin of a tRNA (ser AGN) that Contains a Dihydrouridine Arm Replacement Loop," J. Mol. Evol. 28:374-8.
Harrison, L.J., et al., 1990, "Differential Diagnosis of Taenia saginato and Taenia solium with DNA Probes," Parasitology 100: 459-61.
Kinder, A., et al., 1992, "Salivary and Serum Antibodies in Experimental Canine Taeniasis," Vet. Parasitol. 41:321-7.
McManus, D.P., et al., 1989, "Human Cysticercosis and Taeniasis: Molecular Approaches for Specific Diagnosis and Parasite Identification," Acta Leiden 57:81-91.
MacManus, D.P., et al., 1990, "Characterization of Taeniid Cestodes by DNA Analysis," Rev. Sci. Tech. Off. Int. Epiz. 9:489-510.
Pathak, K.M.L., et al., 1994, "A Western Blot and ELISA Assay for the Diagnosis of Taenia solium Infection in Pigs," Vet. Parasitol. 53:209-17.
Rishi, A.K. and MacManus, D.P., 1987, "DNA Probes which Unambiguously Distinguish Taenia solium from T. saginata," Lancet i:1275-76.
Rishi, A.K., and MacManus, D.P., 1988, "Molecular Cloning of Taenia solium genomic DNA and Characterization of Taeniid cestodes by DNA analysis," Parasitology 97:161-76.
Barker, D.C., "Molecular Approaches to DNA Diagnosis," Parasitology, 1989, vol. 99, pp. S125-S-146.
Chapman, et al., "Isolation and Characterization of Species-Specific DNA Probes form Taenia Solium and Taenia Saginata and Their Use in an Egg Detection Assay," Journal of Clinical Microbiology. May 1995, vol. 33(5), pp. 1238-1288.
Barker, D.C. Parasitology 99:S125-S146, 1989.

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