Specific DNA primers and method to use same detect Eperythrozoon

Details Specific DNA primers and method to use same detect Eperythrozoon Specific DNA primers and method to use same detect Eperythrozoon

1993-01-22

1994-09-27

Parr, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 243, 536 2432, 4352521, C12Q 168

Patent

active

053506723

ABSTRACT:
The use of E. suis specific primers in PCR with DNA from swine blood increases the sensitivity of current DNA hybridization protocols for determining whether swine are infected with E. suis prior to the development of any clinical symptoms. The present invention provides these E. suis primers and a method to use these primers in a PCR protocol to provide a highly sensitive diagnostic assay for early signs of an E. suis infection.

REFERENCES:
Ludwig et al., International J. Systematic Bacteriology, 42(1): 161-167, Jan. 1992.
Beaucage, S. L., et al., "Deoxynucleoside Phosphoramidites-A New Class of Key Intermediates for Deoxypolynucleotide Synthesis," Tetrahedron Letters 22:1859-1862 (1981).
Gwaltney, Sharon M., et al., "Detection of Eperythrozoon suis using the polymerase chain reaction," J. Vet. Diagn. Invest. 5:40-46 (1993).
Hall, S. M., et al., "Isolation of Infective and Non-infective Eperythrozoon suis Bodies From the Whole Blood of Infected Swine, " Vet. Rec. 123:651 (1988).
Hsu, Frank S., et al., "Evaluation of an Ezyme-linked Immunosorbent Assay for Detection of Eperythrozoon suis Antibodies in Swine," Am. J. Vet. Res. 53:352-354 (1992).
Mullis, Kary B., "Specific Synthesis of DNA in vitro via a Polymerase-Catalyzed Chain Reaction," Methods in Enzymology 55:335-350 (1987).
Oberst, R. D., et al., "Recombinant DNA Probe Detecting Eperythrozoon suis in Swine Blood," Am. J. Vet. Res. 51:1760-1764 (1990).
Oberst, R. D., et al., "Detection of Eperythrozoon suis DNA from Swine Blood by Whole Organism DNA Hybridizations," Vet. Microbiol. 24:127-134 (1990).
Ou, Chin-Yih, et al., "DNA Amplification for Direct Detection of HIV-1 DNA of Peripheral Blood Mononuclear Cells," Science 239:295-297 (1988).
Panaccio, et al., "PCR Based Diagnosis in the Presence of 8% (v/v) Blood," Nucleic Acids Research 19:1151 (1991).
Saiki, Randall K., "Primer-Directed Enzymatic Amplification of DNA With a Thermostable DNA Polymerase," Science 239:487-491 (1988).
Sanger, F., et al., "DNA Sequencing With Chain-Terminating Inhibitors," Proc. Natl. Acad. Sci. USA 74:5463-5467 (1977).
Smith, A. R., et al., "An Indirect Hemagglutination Test for the Diagnosis of Eperythrozoon suis Infected in Swine," Am. J. Vet. Res. 36:1319-1321 (1975).
Southern, E. M., "Detection of Specific Sequences Among DNA Fragments Separated by Gel Electrophoresis," J. Mol. Biol. 98:503-517 (1975).
Welsh, J., et al., "Fingerprinting Genomes Using PCR with Arbitrary Primers," Nucleic Acids Res. 18:7213-7218 (1990).
Zoig, J. W., et al., "High Salt Lysates: A Simple Method to Store Blood Samples Without Refrigeration for Subsequent Use With DNA Probes," Am. J. Trop. Med. Hyg. 39(1):33-40 (1988).

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