Specific binding assays utilizing analyte-cytolysin conjugates

Details Specific binding assays utilizing analyte-cytolysin conjugates Specific binding assays utilizing analyte-cytolysin conjugates

1982-10-20

1985-05-14

Marantz, Sidney

Chemistry: analytical and immunological testing

Biospecific ligand binding assay

435 4, 435 5, 435 7, 435 21, 435 36, 436512, 436520, 436541, 436803, 436813, 436815, 436817, 436827, 436828, 436829, G01N 3354, G01N 3350, G01N 3374

Patent

active

045173036

ABSTRACT:
A novel analyte-cytolysin conjugate and its use in a lipid vesicle mediated measurement process is described for a wide variety of analytes present at very low concentration. The method involves forming a reaction system consisting of analyte, analyte specific binding agent, analyte-cytolysin conjugate, and vesicles containing detectable marker material in such proportions that uncombined conjugate alters the permeability of the vesicles resulting in the release and quantitative detection of marker material which can be correlated with the amount of analyte initially present.

REFERENCES:
patent: 4235792 (1980-11-01), Hsia et al.
patent: 4342826 (1982-08-01), Cole
patent: 4372745 (1983-02-01), Mandle
Chemical Abstracts, 94:43718y (1981).
K. Uemura et al., Jour. of Immunological Methods, 53, 221-232 (1982).
Haga et al., Liposome Immunosensor for Theophylline, Biochem., Biophys. Res. Commun., vol. 95, 187-192 (1980).
Haga et al., Drug Sensor: Liposome Immunosensor for Theophylline, Anal. Biochem., vol. 118, 286-293 (1981).
Hsia and Tan, Membrane Immunoassay, New York Acad. Sci., vol. 308, 139-148 (1978).
Thelestam and Mollby, Plant and Animal Cytolysins, Biochem. Biophys. Acta, vol. 557, 156-169 (1979).
Sessa et al., Interaction of a Lytic Polypeptide, Meliltin, with Lipid Membrane Systems, J. Biol. Chem., vol. 244, 3575 (1969).

Affiliated with

Also associated with

Rating

Specific binding assays utilizing analyte-cytolysin conjugates does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Specific binding assays utilizing analyte-cytolysin conjugates, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Specific binding assays utilizing analyte-cytolysin conjugates will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-768916