Specific binding assay with an enzyme modulator as a labeling su

Details Specific binding assay with an enzyme modulator as a labeling su Specific binding assay with an enzyme modulator as a labeling su

1976-12-06

1979-01-16

Tanenholtz, Alvin E.

Textiles: fiber preparation

1951035A, 424 12, G01N 3300, G01N 3114

Patent

active

041347927

ABSTRACT:
A specific binding assay method employing, as a labeling substance, a reversibly binding enzyme modulator for the detection of a ligand in a liquid medium. The method follows conventional specific binding assay techniques of either the homogeneous or heterogeneous type wherein the liquid medium to be assayed is combined with reagent means that includes a labeled conjugate to form a binding reaction system having a bound-species and a free-species of the conjugate. The amount of conjugate resulting in the bound-species or the free-species is a function of the amount of ligand present in the liquid medium assayed. In the present invention, the labeled conjugate comprises a reversibly binding enzyme modulator covalently linked to a binding component of the binding reaction system. The distribution of the conjugate between the bound-species and the free-species is determined by addition of an enzyme whose activity is affected, either decreased or increased, by said modulator and measuring the resulting activity of the enzyme. The enzyme modulator may be a conventional enzyme inhibitor, preferably of the coompetitive type, or an allosteric effector.

REFERENCES:
patent: 3817837 (1974-06-01), Rubenstein et al.
patent: 3850752 (1974-11-01), Schuurs et al.
patent: 3880934 (1975-04-01), Rammler
patent: 3975342 (1976-08-01), Gross
patent: 4003792 (1977-01-01), Mill et al.
Miles et al., Nature, vol. 219, (1968), pp. 186-189.
Landon, "A Suggested Terminology for Non-Isotopic Immunoassays", Conference on Non-isotopic Immunoassays, (1976).

Affiliated with

Also associated with

Rating

Specific binding assay with an enzyme modulator as a labeling su does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Specific binding assay with an enzyme modulator as a labeling su, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Specific binding assay with an enzyme modulator as a labeling su will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-650328