Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1996-07-17
1999-06-29
Duffy, Patricia A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 792, 435 12, 435 17, 435 21, 435 25, 435 26, 435 28, 436518, 436523, 436524, 436528, 436533, 436534, G01N 3353
Patent
active
059167572
ABSTRACT:
Specific binding ligands can be detected with an assay which utilizes an immobilized receptor for the ligand, an immobilized reporter enzyme, an inhibitor antibody and a a water-soluble conjugate of the ligand and an anti-inhibitor antibody. Both antibodies are specific for the reporter enzyme, but the antibodies affect enzymatic activity differently. The inhibitor antibody effectively shuts down the activity of the reporter enzyme when it is complexed thereto. The anti-inhibitor antibody binds to the reporter enzyme, does not affect the enzymatic activity, but prevents the binding of the inhibitor enzyme. This assay provides a direct correlation of the generated signal to the target specific binding ligand.
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Contestable Paul B.
Daiss John L.
Grogan Elizabeth A.
Groth Holly L.
Snyder Brian A.
Duffy Patricia A.
Johnson & Johnson Clinical Diagnostics Inc.
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