Specific binding assay employing an enzyme-cleavable substrate a

Details Specific binding assay employing an enzyme-cleavable substrate a Specific binding assay employing an enzyme-cleavable substrate a

1979-10-23

1981-07-21

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 18, 435 19, 435188, 435810, 23230B, 424 8, 424 12, 252408R, G01N 3354, C12Q 134, C12Q 144, C12N 996

Patent

active

042799929

ABSTRACT:
An improved specific binding assay method and reagent for determining a ligand in a liquid medium employing, as an enzyme-cleavable substrate label, a residue having the formula:

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patent: 4171432 (1979-10-01), Carrico et al.
patent: 4226978 (1980-10-01), Boguslaski et al.
Furth et al., "Specificity and Multiple Forms of .beta.-Galactosidase in the Rat", Biochem. J., vol. 97, (1965) pp. 59-66.
Burd et al., "Specific Protein-Binding Reactions Monitored by Enzymatic Hydrolysis of Ligand-Fluorescent Dye Conjugates", Anal. Biochem., vol. 77, (1977), pp. 56-67.
Schuurs et al., "Enzyme-Immunoassay", Clin. Chim. Acta, vol. 81, No. 1, (1977), pp. 1-40.
Sercarz et al., "Antigen Binding to Cells: Determination by Enzymic Fluorgenic Group Hydrolysis", Science, vol. 159, No. 3817, (1968), pp. 884-885.

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