Specific binding assay conjugate with spaced-apart receptors

Details Specific binding assay conjugate with spaced-apart receptors Specific binding assay conjugate with spaced-apart receptors

1982-11-29

1986-03-25

Marantz, Sidney

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

260112R, 436500, 436531, 436546, 436547, 436815, G01N 3354

Patent

active

045783608

ABSTRACT:
In the immunoassay of antigens in liquids, a reaction mixture is formed containing the liquid under assay, labelled antigen, and a mixed binding reagent which contains an antigen-binding site and a label-binding site, the two sites being spaced apart in the reagent so that a single molecule of labelled antigen cannot bind to both sites. The label is one whose activity is changed upon binding to a label-binding site, and the amount of antigen in the original liquid sample is determined by measuring the activity of the label in the reaction mixture. A preferred label is a fluorophore. The mixed binding reagent preferably consists of two antibodies linked together.

REFERENCES:
patent: 4446233 (1984-05-01), Auditore-Hargreaves
patent: 4474893 (1984-10-01), Reading
V. Ghetie et al., Molecular Immunology, 17, 395-401, (1980).
Chemical Abstracts, 78:14332g, (1973).
A. Nisonoff, Arch. Biochem. Biophys, 93(2), 460-462, (1961).

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