Specific binding assay compound with inhibitive self-quenching c

Details Specific binding assay compound with inhibitive self-quenching c Specific binding assay compound with inhibitive self-quenching c

1989-11-29

1995-01-24

Scheiner, Toni R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 2532, 536 111, 435 71, 435968, 436544, 436545, 436546, 436800, 436805, 436827, 436828, 530802, 5303913, C07H 2100, G01N 3353, C12Q 168

Patent

active

053842413

ABSTRACT:
Disclosed is an assay system including a compound comprising an analyte-specific moiety having substituted thereon a polymer comprising plurality of self-quenching emitter moieties and a plurality of isocharged functionality separating the emitter moieties. The present invention provides compounds that overcome the undesirable effects of self-quenching when multiple emitter moieties are used for labelling of assay reagents. Avoidance of this self-quenching phenomenon by the compounds of the invention makes it possible to introduce a more concentrated degree of labelling on to analyte-specific molecules such as oligo nucleotide probes, antibodies and other specific binding proteins and analyte-specific polysaccharides. Therefor, it is possible to effect greater assay sensitivity because the number of labels per recognition molecule(analyte-specific moiety) can be increased beyond the point previously possible without the reduction in signal caused by self-quenching.

REFERENCES:
patent: 3996345 (1976-12-01), Ullman et al.
patent: 4134792 (1979-01-01), Boguslaski et al.
patent: 4166105 (1979-08-01), Hirschfeld
patent: 4213893 (1980-07-01), Carrico et al.
patent: 4230797 (1980-10-01), Boguslaski et al.
patent: 4238195 (1980-12-01), Boguslaski et al.
patent: 4302204 (1981-11-01), Wahl et al.
patent: 4318980 (1982-03-01), Boguslaski et al.
patent: 4318981 (1982-03-01), Burd et al.
patent: 4318982 (1982-03-01), Hornby et al.
patent: 4358535 (1982-11-01), Falkow et al.
patent: 4395486 (1983-07-01), Wilson et al.
patent: 4442204 (1984-04-01), Greenquist et al.
patent: 4452886 (1984-06-01), Henry
patent: 4492751 (1985-01-01), Boguslaski et al.
patent: 4556643 (1985-12-01), Paau et al.
patent: 4563417 (1986-01-01), Albarella et al.
patent: 4581333 (1986-04-01), Kourilsky et al.
patent: 4748111 (1988-05-01), Dattagupta et al.
patent: 4849513 (1989-07-01), Smith et al.
patent: 4876335 (1989-10-01), Yamane et al.
patent: 5093232 (1992-03-01), Urdea et al.
patent: 5118800 (1992-06-01), Smith et al.
Haralambidis, et al., Nuc. Acids Research, vol. 15, No. 12, pp. 4856-4876, (1987).
Visor, et al, Journal of Pharmaceutical Sciences, vol. 70, No. 5 pp. 469-475 (1981).
Soini, et al, Clin. Chem., vol. 25, No. 3 pp. 353-361 (1979).
Exley, et al., J. Steroid Biochem., vol. 14 pp. 1297-1302 (1981).
Richardson, et al., Nuc. Ac. Res., vol. 11, No. 18, pp. 6167-6184 (1983).
Hemmila, Clin. Chem., vol. 31, No. 3, pp. 359-370 (1985).
Hassan, et al, FEBS Letters, vol. 103, No. 2, pp. 339-341 (1979).
Haralambidis, J., et al., Chemical Abstracts, vol. 108 (21), abstract No. 187182z (1987).

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