Specific binding assay

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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Details

435 14, 435 25, 435188, G01N 3354, C12Q 126, C12Q 154, C12N 996

Patent

active

044736380

ABSTRACT:
An improvement in a specific binding assay for determining a ligand in a liquid medium, in which assay the labelled conjugate includes FAD and the ligand and in which the labelled conjugate can bind to apoglucose oxidase to form a holoenzyme is provided. The improvement, which renders the assay practical at 37.degree. C., comprises carrying out the assay in the presence of at least about a 250 mM concentration of a polyhydric aldehyde or alcohol, including substrate.

REFERENCES:
patent: 4366243 (1982-12-01), Rupchock

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