Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1982-04-22
1984-09-25
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 14, 435 25, 435188, G01N 3354, C12Q 126, C12Q 154, C12N 996
Patent
active
044736380
ABSTRACT:
An improvement in a specific binding assay for determining a ligand in a liquid medium, in which assay the labelled conjugate includes FAD and the ligand and in which the labelled conjugate can bind to apoglucose oxidase to form a holoenzyme is provided. The improvement, which renders the assay practical at 37.degree. C., comprises carrying out the assay in the presence of at least about a 250 mM concentration of a polyhydric aldehyde or alcohol, including substrate.
REFERENCES:
patent: 4366243 (1982-12-01), Rupchock
E. I. Du Pont de Nemours and Company
Kepplinger Esther M.
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