Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-03-06
1999-06-01
Degen, Nancy
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 4, 4351721, 4352541, 4352551, 4353201, 536 231, 536 241, C12Q 168, C12Q 102, C07H 2104, C12N 1581
Patent
active
059087480
ABSTRACT:
An extended species-specific sequence region of yeast transcription factor IIB (yTFIIB) (amino acids 144-157) is critical for cell viability and gene activation. Four residues in yTFIIB, Lys-147, Cys-149, Lys-151, and Glu-152, are critical for yeast cell growth. Intragenic suppressor experiments identified mutations that reversed, or partially reversed, the temperature sensitive phenotype of a yTFIIB derivative bearing amino acid changes at these four positions to human residues. Some of these residues (positions 115, 117 and 182) are located outside the species-specific region of yTFIIB. The identification of this species-specific region provides a unique and specific target for antifungal drugs and a screening assay therefor.
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Carson Daniel J.
Dorsey Michael J.
Ma Jun
Shaw Shruti
Wingfield Jonathan
Children's Hospital Medical Center
Degen Nancy
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