Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Reexamination Certificate
2008-07-22
2008-07-22
Horlick, Kenneth R. (Department: 1637)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
C435S006120, C435S091500, C536S024320, C536S024330
Reexamination Certificate
active
11318907
ABSTRACT:
The inventions provides a method for identifying a target virus in an infected subject comprising the steps of designing a pair of degenerate primers corresponding to highly conserved regions of the target virus; designing a pair of species-specific primers according to highly variable sequences within the conserved regions of the target virus; preparing the species-specific probes according to the larger sequence variations within the conserved regions of the target virus, which are amplified with the species-specific primers as obtained; preparing a test sample by amplifying total nucleic acid of the infected subject with the degenerate primers as obtained; contacting the test sample with the species-specific probes as obtained; and detecting a hybridization between the species-specific probe and the test sample, wherein the hybridization indicates the target virus is identified in the infected subject. The primers and probes for detecting a garget virus are also provided.
REFERENCES:
patent: 2003/0134293 (2003-07-01), Liu
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Chang Ya-Chun
Hsu Yueh-Chwen
Yeh Tzu-Jung
Bertagna Angela
Bureau of Animal and Plant Health Inspection and Quarantine
Horlick Kenneth R.
Panitch Schwarze Belisario & Nadel LLP
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