Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-08-28
1999-02-23
Horlick, Kenneth R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 536 221, 536 243, 536 253, 536 2433, C12P 1934, C07H 2104, C07H 2100
Patent
active
058742212
ABSTRACT:
Phythophthora species which infect potatoes may result in the devastating disease potato late blight or in pink rot. Primers specific for Phythophthora infestans (late blight), and for Phytophthora erythroseptica and Phytophthora nicotianae (pink rot) have been designed which are useful for detecting the presence of the microorganisms by polymerase chain reaction methods. The primers were derived from the internal transcribed spacer region of Phytophthora ribosomal DNA and may be used to confirm the presence of the microorganisms or to distinguish among them.
REFERENCES:
Ersek et al., "PCR Amplification of Species-Specific DNA Sequences Can Distinguish Among Phytophthora Species", Applied and Environmental Microbiology, vol. 60(7), pp. 2616-2621, Jul. 1994.
Niepold et al., "Application of the PCR Technique to Detect Phytophthora infestans in Potato Tubers and Leaves", Microbiol. Res., vol. 150, pp. 379-385, 1995. (Only Abstract).
Niepold et al. Application of the PCR technique to detect phytophthora infestans in potato tubers and leaves. Microbiological Research vol. 150(4), pp. 379-385. (Only Abstract), 1995.
O'Donnell Ribosomal DNA internal transcribed spacers are highly divergent in the phytopathogenic ascomycete Fusarium sambucinum. Curr. Genet. vol. 22, pp. 213-220, 1992.
Wildman et al. Studies on the secondary structure of 5.8S rRNA from a Thermophile, Thermomyces Ianuginosus. J. Biol. Chemistry. vol. 256, pp. 5675-5682, 1981.
Tooley et al. Development of PCR primers from internal transcribed spacer region 2 for detection of Phytophthora species infecting potatos. Applied and Environmental Microbiology, vol. 63(4), pp. 1467-1475, 1997.
Database search Accession NO: Y08674 and Y08670, 1997.
Bunyard Britt
Carras Marie
Hatziloukas Efstathios
Tooley Paul
Fado John
Graeter Janelle S.
Horlick Kenneth R.
Silverstein M. Howard
The United States of America as represented by the Secretary of
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