Species-specific DNNA probe for the detection of Branhamella cat

Details Species-specific DNNA probe for the detection of Branhamella cat Species-specific DNNA probe for the detection of Branhamella cat

1989-03-30

1991-07-09

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 4, 435 29, 436 94, 436501, C12Q 100, C12Q 168

Patent

active

050305565

ABSTRACT:
There is provided by the present invention, a method for detecting the presence of Branhamella catarrhalis, said method comprising; depositing and fixing on an inert support a sample containing DNA fragments in substantially single stranded form, contacting said fixed single stranded genetic material with a labelled probe consisting of a fragment of B. catarrhalis chromosomal DNA, identified as ATCC 53879, being capable of hybridizing with B. catarrhalis DNA in the sample, said contacting being under hybridizing conditions at a pre-determined stringency; and detecting duplex formation on said support by means of said label.

REFERENCES:
patent: 4358535 (1982-11-01), Folkow et al.
Beaulieu, D. et al., "Characterization, Use of as a Molecular Probe . . . " , Abstr. Annu. Meet. Am. Soc. Microbiol, vol. 87(0), p. 149 (1987).
Beaulieu, D. et al., "Characterization of a Plasmid from . . . " Plasmid, vol. 20, pp. 158-162 (1988).
Knapp, "Historical Perspectives and Identification of Neisseria and Related Species", Oct. 1988, vol. I, No. 4, pp. 415-431.
CA 221758w--Rossau et al.
CA 12543h--Van Embden et al.

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