Species-specific detection of Mycobacterium kansasii

Details Species-specific detection of Mycobacterium kansasii Species-specific detection of Mycobacterium kansasii

1996-06-14

1997-03-25

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 536 243, C12P 1934

Patent

active

056143907

ABSTRACT:
The present invention provides oligonucleotides useful as amplification primers and assay probes for species-specific detection and identification of Mycobacterium kansasii. The oligonucleotides of the invention are derived from the p6123 sequence previously reported by Yang, et al. (1993) and amplify a target sequence of both atypical and typical M. kansasii strains, allowing detection and identification of a broad range of M. kansasii strains. The oligonucleotides may be used after culture as a means for confirming the identity of the cultured organism or they may be used prior to culture or in place of culture for detection and identification of mycobacterial nucleic acids using a variety of amplification methods.

REFERENCES:
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patent: 5500341 (1996-03-01), Spears
patent: 5518884 (1996-05-01), Spears et al.
B. C. Ross, et al. "Identification of a Genetically Distinct Subspecies of Mycobacterium kansasii" J. Clin. Microbiol. 30:2930-2933 (1992).
B. Boddinghaus, et al. "Detection and Identification of Mycobacteria by Amplification of RNA" J. Clin. Microbiol. 28:1751-1759 (1990).
T. Rogall, et al., "Differentiation of Mycobacterium species by direct sequencing of amplified DNA" J. Gen. Microbiol. 136:1915-1920 (1990).
Z. H. Huang, et al. "Identification of Mycobacterium kansasii by DNA Hybridization" J. Clin. Microbiol. 29:2125-2129 (1991).
E. Tortoli, et al. "Evaluation of a Commercial DNAN Probe Assay for the Identification of Mycobacterium kansasii" Eur. J. Clin. Microbiol. Infect. Dis. 13:264-267 (1994).
M. Yang, et al. "Isolation of a DNA Probe for Identification of Mycobacterium kansasii, including the Genetic Subgroup" J. Clin. Microbiol. 31:2769-2772 (1993).

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