Species-specific detection of Mycobacterium kansasii

Details Species-specific detection of Mycobacterium kansasii Species-specific detection of Mycobacterium kansasii

1994-09-19

1996-03-19

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 536 2432, 536 2433, 935 8, 935 17, 935 77, 935 78, C12Q 168, C12P 1934, C07H 2104

Patent

active

055003419

ABSTRACT:
M. kansasii-specific oligonucleotide amplification primers and methods for detecting and identifying M. kansasii nucleic acids using the amplification primers. One hundred percent of the clinical and environmental M. kanasii isolates tested were positive in amplification assays using the inventive amplification primers, with no cross-reactivity in other species of mycobacteria or closely related non-mycobacteria.

REFERENCES:
Yang et al, J. Clin Microbiol (1993) 31:2769-2722.
T. Rogall et al Differentiation of Mycobacterium Species by Direct Sequencing of Amplified DNA J. of General Microbiology (1990) 136, 1915-20.
B. Boddinghaus et al Detection and Identification of Mycobacteria by Amplification of rRNA J. of Clinical Microbiology (Aug. 1990) 1751-59.
B. Ross et al Identification of a Genetically Distinct Subspecies of Mycobacterium kansasii J. of Clinical Microbiology (Nov. 1992) 2930-33.
M. Yang et al Isolation of a DNA Probe for Identification of Mycobacterium kansasii Including the Genetic Subgroup J. of Clinical Microbiology (Oct. 1993) 2769-72.
Z. Huang et al Identification of Mycobacterium kansasii by DNA Hybridization J. of Clinical Microbiology (Oct. 1991) 2125-29.

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